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Mebiol® Gel

Mebiol Gel
Cat. No.
Intended use
Stem cell and pluripotent stem cell culture, expansion, and differentiation
Spheroid culture
Cell implantation
Organ and Tissue Regeneration
Drug Delivery
Non-cell culture application
The thermoreversible gelation polymer, “Mebiol Gel” has been developed as proprietary hydrogel. Mebiol Gel has been commercialized as a cell/tissue culture reagent for ES cells, chondrocytes and cancer cells, etc. Mebiol Gel based intralumenal implants are being developed for occlusion of cancerous vascular system and brain aneurism.

Features of Mebiol Gel
An aqueous solution of Mebiol Gel is fluid liquid (sol state) at low temperatures (0°C - 15°C), however, it turns into anelastic hydrogel (gel state) at temperatures higher than room temperature (25°C). It is possible to mix it with various drugs or culture medium at the sol state. The sol-gel transformation of Mebiol Gel occurs fully thermoreversible. Elasticity ofthe hydrogel increases with temperature increase and is appropriate for three-dimensional culture of cells /tissues at around 37°C. cells /tissues in the gel is clearly observed through optical microscope during cultivation at 37°C owing to great transparency of Mebiol Gel. Fibroblast is alive but does not grow in Mebiol Gel therefore other aiming cellscan begrown selectively. Cultured cells /tissues can be recovered easily from Mebiol Gel by lowering temperature without any damage on cells /tissues.

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Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Mebiol Gel
Figure Explain:
IMG1. Thermoreversible Sol-Gel transition of Mebiol® Gel
IMG2. Cancerous human colon tissue cultured in Mebiol® Gel for 10 days. (Courtesy of Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine)
IMG3. Suppression of Fibroblast Growth in Mebiol® Gel
IMG4. Macaca embryonic stem cells cultured on 2D culture on Feeder cells
IMG5. Macaca embryonic stem cells cultured on Mebiol® Gel without LIF show morphology and alkaline phosphatase staining characteristic of undifferentiation. (Day 7)
IMG7. 3D Culture of Mouse Embryonic Stem (ES) Cells in Mebiol® Gel (without LIF, 5days)
IMG8. Low temperature: Fluid Liquid (sol state) when cooled to 15°C
IMG9. High temperature: Elastic Hydrogel (gel state) at culture temperature (37°C)
IMG10. Preparation step of Mebiol® Gel
Citations and References