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GenomONE™-Neo EX HVJ-E 1 vial Transfection Reagents

GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
Cat. No.
ISK-GN-001-EX
Quantity
1SET
Price
¥ 25,000
$ 334
€ 250
Inventory
Available
Intended use
GenomONETM -Neo EX is a non-viral reagent for transfection, Unique, efficient transfection of siRNA, DNA oligonucleotides, proteins. Low toxicity with primary cells. Protocols for in vivo transfection.
Composition
Freeze-dried HVJ-E (inactivated HVJ) - 1 vial (0.26 mL /via)
Reagent A (enhancer for incorporation) - 1 vial (0.5 mL/vial)
Reagent B (reagent for incorporation) - 1 vial (0.3 mL/vial)
Reagent C (enhancer for introduction) - 1 vial (1.0 mL/vial)
Buffer (for suspension and dilution) - 1 vial (6.5 mL/vial)
Other
[Background]
HVJ (hemagglutinating virus of Japan) Envelope VECTOR KIT is a tool for transfection of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cells and animal tissue by means of membrane fusion. The HVJ envelope, carrying the molecule to be transfected, is composed of a completely inactivated and purified HVJ (Sendai virus) while preserving the cell membrane-fusing capability of the envelope.
GenomONE provides ready-to-use kits containing the HVJ envelope and auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, buffer).

[Principle]
What is HVJ Envelope (HVJ-E)
HVJ Envelope is a purified product prepared through complete inactivation of Sendai virus (HVJ#).
It is a vesicle in which only the cell membrane-fusing capability of the envelope protein of Sendai virus is retained.
It is known that the HN protein in the tunica externa of the Sendai virus recognizes the receptor (possessing sialic acid atthe terminal of sugar chain) on the cell membrane and adsorbs it, leading to the induction of membrane fusion mediated by F protein (another component of the envelope). The genomic RNA of the Sendai virus contained in HVJ-E has been inactivated completely and has neither infective nor proliferative potentials in humans or experimental animals. HVJ-E can be used safely at ordinary laboratories, without requiring any special operations or facilities.
#HVJ: Hemagglutinating Virus of Japan.
Conventionalnon-viral transfection tools, including cationic lipids, are incorporated into cells through endocytosis which results in degradation of the transferred DNA by lysosomes. On the other hand, HVJ Envelope VECTOR resists degradation by lysosomes, makingit easy to transfer the specified DNA. Therefore, HVJ Envelope VECTOR yields highly efficient gene expression. Sialic acid receptors, which are needed to trigger binding with HN protein, exist in almost all animal cells. Thus, HVJ EnvelopeVECTOR isuseful for a wide range of targets.

[Feature and Advantages]
1. Wide usability - GenomONETM HVJ Envelope VECTOR KIT is a highly flexible tool for transfecting a wide variety of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins,antibodiesetc.) into cultured cells (adherent and non-adherent) and tissue. GenomONE is useful for transfecting sensitive primary cells and is further distinguished by its ability to deliver contents into live animals. Many literature citations areavailable foreach GenomONE-Neo application.
2. Safety - Unlike cationic lipids, GenomONE-Neo delivers the molecule of interest into cells via membrane fusion, not by endocytosis where cargo may be degraded by lysosomal enzymes. Since GenomeONETM-Neo is apurified HVJ (Sendai virus) product, prepared from virus particles completely inactivated for infectious ability and proliferative potential it is completely safe to use without special precautions.
3. Easy - GenomONETM provides ready-to-use kitscontaining the HVJ envelope and required auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, and buffer). Suggested protocols for all major applications are included.

[Application]
1. siRNA transfection
in vitro:
Primary T cell (Human peripheral blood)
Primary cardiac myocyte (Rat cardiac myocyte)
Differentiated C2C12 (Mouse myoblast)
monkey ES cells
Min6 (Mouse pancreatic (BETA) cell)
U937 (Human myelomonocytic cell)
in vivo:
Intradermally transplanted human cervix cancer /HeLa (SCID mouse)
Submandibular gland (rat)
2. Protein delivery
in vivo:
Primary macrophage (C3H mouse peritoneal resident)
Swiss 3T3 cell (Mouse embryonic fibroblasts)
in vivo:
Nucleus tractus solitarius (Rat brain)
3. Oligo DNA transfection
in vitro:
Primary HDMECs (Human dermal microvascular endothelial cell)
Primary CD34+ cell (Human blood)
in vivo:
Uterus (Mouse)
Skin, ear lobe(Mouse)
Lung (Mouse)
4. Plasmid DNA transfection
in vivo:
Uterus(Mouse)
Palatal periodontal tissue (Rat)
Myocardium (Rat heart)
Lung (neonatal porcine)
Subcutaneously transplanted human colon cancer /LoVo (nude mouse)
Storage
4C
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
Figure Explain:
IMG6. 1: Non-treated, 2: PLB siRNA (10µg ), 3: PLB siRNA (2µg), 4: Scramble siRNA (10µg), 5: HVJ-E only
IMG2. 1: PLB siRNA (10µg ), 2: PLB siRNA (2µg), 3: Scramble siRNA (10µg ), 4: Non-treated
Reference
References