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Products

PUREfrex®2.0

PUREfrex2.0
Cat. No.
GFK-PF201-0.25-EX
Quantity
1KIT
(for 250µL reaction (5 x 25µL reactions))
Price
¥ 15,000
$ 200
€ 150
Inventory
Available
Composition
for 250µL reaction (5 x 25µL reactions)
• Solution I (White) 125µL
• Solution II (Black) 12.5µL
• Solution III (Red) 12.5µL x 2
• DHFR DNA (Clear) 10µL
Other
Background
PUREfrex™ kit is a newly developed reconstituted cell-free protein synthesis reagent based on PURE system technology invented by Professor Takuya Ueda in the University of Tokyo. This reaction systemconsists of proteins, ribosome, amino acids and NTPs only (Ref. 1, 2). Those proteins are necessary for transcription, translation and energy regeneration. The proteins and ribosome are highly purified individually and assembled together to constitutethe protein synthesis system. To synthesize your protein, just add your template DNA or mRNA encoding the protein of interest into reaction mixture, and incubate for several hours. This system’s biggest point is RECONSTITUTED system by assembling translation related factors only. By this unique character, you may adjust the composition of reaction mixture as you like and may not have to consider serious background for your downstream application.
By improving the purification process of components of PUREfrex™ kit, contamination of RNase and β-galactosidase are greatly reduced, in addition to that, lipopolysaccharide (LPS) is also reduced less than 0.1 EU per 1 µL of reaction mixture. All proteinous components of PUREfrex™ kit have no tags for purification and detection. It allows to fuse your protein with any tag to purify the product.

Term of a guarantee: 4 months
Storage
-70C
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Figure Explain:
IMAGE5: Outline of protein synthesis in the PUREfrex