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Products

OKD Oxidative Stress Detector

Cat. No.
KAL-STDTC-3
Quantity
5UG
Price
¥ 125,000
$ 1667
€ 1250
Inventory
Available
Composition
Plasmid DNA
Other
[Background]
Cellular stresses, which are implicated in various diseases, include stresses caused by intra-tissue factors such as low oxygen status and intracellular stresses such as endoplasmic reticulum (ER) stress and oxidative stress. Oxidative stress conditions enhance the production of reactive oxygen species and are associated with various human diseases, including neurodegenerative disorders, inflammation and various cancers.
OKD Oxidative Stress Detector utilizes the expression regulation system of Nrf2 transcriptional factor. Under normal condition, Nrf2 is rapidly degraded by the ubiquitin-proteasome pathway through the association with Keap1. Upon exposure to oxidative stress, reactive cysteine residues in Keap1 are covalently modified, leading to the liberation of Nrf2 from Keap1-mediated degradation. The stabilized Nrf2 is then translocated to the nucleus, and activates the transcription of a wide range of oxidative stress responsive genes.

OKD Oxidative Stress Detector is a reporter gene in which luciferase gene is fused with Nrf2 cDNA corresponding to ubiquitination domain. In cells introduced with this detector gene, oxidative stress stabilizes the luciferase fusion protein and induce the luciferase activity.
For detection of oxidative stress in living mouse, Tg type OKD-Luc mouse which is introduced with ERAI ER Stress Detector is suitable.
Storage
-20C
Reference
  • Oikawa D., Akai R., Tokuda M., Iwawaki T. Sci. Rep. 2, 229 (2012) PMID: 22355743