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ERAI ER Stress Detector

Cat. No.
KAL-STDTC-1
Quantity
5UG
Price
¥
Inventory
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Composition
Plasmid DNA
Other
[Background]
Cellular stresses, which are implicated in various diseases, include stresses caused by intra-tissue factors such as low oxygen status and intracellular stresses such as endoplasmic reticulum (ER) stress and oxidant stress. ER stress is caused by the accumulation of unfolded proteins in the ER lumen and associated with neurodegenerative diseases, metabolic syndrome, and various cancers.
ERAI ER Stress Detector and Detector HD utilizes the expression regulation system of ER stress factor XBP1 protein. XBP1 mRNA is transcribed with intron sequence, which is translated as inactive XBP1 protein. However, under the ER stress condition, intron is spliced out, and active XBP1 protein is produced. ERAI ER Stress detector is a reporter gene in which luciferase gene is connected to downstream of intron region of XBP1 cDNA. In cells introduced with this detector gene, ER stress induces splicing of the XBP1 intron sequence, and fusion protein of XBP1 N terminal region and luciferase is translated.

 ERAI ER Stress Detector      : 
Broad range
 ERAI ER Stress Detector HD :  High sensitivity
Storage
-20C
Reference
  • Iwawaki T., Akai R., Yamada S., Kohno K. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009) PMID: 19805353