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2-Deoxyglucose (2DG) Uptake Measurement Kit

2-Deoxyglucose (2DG) Uptake Measurement Kit
Cat. No.
CSR-OKP-PMG-K01E
Quantity
1KIT
(50 tests)
Price
¥ 58,100¥ 83,000
$ 1107
€ 830
Inventory
Available
Intended use
Direct measurement of 2DG6P without the use of radioisotope.
Composition
Solution A: 3,400 ul x 1 tube
Solution B (Acid solution): 1,000 ul x 1 tube
Solution C (Acid neutralizing solution): 1,000 ul x 1 tube
Solution D: 1,600 ul x 1 tube
Solution E (Alkali solution): 1,000 ul x 1 tube
Solution F (Alkali Neutralizing solution): 1,000 ul x 1 tube
Solution G: 2,000 ul x 1 tube
1 mM 2DG6P solution: 500 ul x 1 tube
Sample diluent buffer Concentrate (100-fold concentrated solution): 3 ml x 1 tube
Substrate buffer: 11 ml x 1 vial
DTNB Substrate (powder): 5 vials
Low G6PDH: 25 ul x 1 tube
High G6PDH: 250 ul x 1 tube
GR: 20 ul x 1 tube
Other
[Background]
Measurement of 2-deoxyglucose (2DG) uptake in tissues and cells is a reliable approach with which to estimate glucose uptake and thereby to explore the regulation of glucose metabolism and mechanism of insulin resistance. Radioisotope-labeled 2DG is usually used for the measurement of 2DG uptake both in vivo and in vitro. However the radioisotope (RI) method is required a specialized facility for RI in strict limitation and cannot be handled in ordinal laboratories. Furthermore, radioactive 2DG administered into cultured cells remains in the extracellular space, and therefore the results must be corrected by separating the extracellular 2DG and intracellular 2DG/2DG-6-phosphate (2DG6P) in the cells.
Thiskit is based on theenzymatic method for the direct measurement of 2DG6P amount without any use of radioisotope materials (Saito K and Minokoshi Y, et al. Analytical Biochem 412: 9-17, 2011).
[Advantages]
1. No RI materials are required, and 2DG uptake can be measured in any ordinal laboratories.
2. Direct measurement of 2DG6P amount accumulated in target cells.
3. High sensitivity with the use of enzyme-recycling amplification reaction.
Storage
-20C
2-Deoxyglucose (2DG) Uptake Measurement Kit
2-Deoxyglucose (2DG) Uptake Measurement Kit
2-Deoxyglucose (2DG) Uptake Measurement Kit
2-Deoxyglucose (2DG) Uptake Measurement Kit
2-Deoxyglucose (2DG) Uptake Measurement Kit
2-Deoxyglucose (2DG) Uptake Measurement Kit
Reference
Saito K, Lee S, Shiuchi T, Toda C, Kamijo M, Inagaki-Ohara K, Okamoto S, Minokoshi Y., Anal Biochem. 2011 May 1;412(1):9-17. PMID: 21262191
Jorgensen BM, Rasmussen HN., Anal Biochem. 1979 Nov 1;99(2):297-303. PMID: 42325

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