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Tear Mucin Assay Kit (O-Glycan Assay Method)

Tear Mucin Assay Kit (O-Glycan Assay Method)
Cat. No.
¥ 96,000
$ 1280
€ 960
Elution Buffer 30mL x 1 vial
Slurry for Spin Column 45mL x 1 vial
Standard Solution (GalNAc 100ug/mL) 1.0mL x 1 vial
Reagent A 0.3mL x 1 vial
Reagent B 1.5mL x 1 vial
Stop Solution 15mL x 1 vial
Empty column For 1mL x 50pc
Centrifuge tube 50pc
Mucins are major components in tear fluid and apical cell membranes on the ocular surface epithelia. Structurally, they are composed of tandem repeat domains containing heavily O-glycosylated serine and threonine residues. More than a half of its weight consists of O-glycans, which has hydrophilic nature. The heavy glycosylation of mucins is believed to impart a highly negative charge and a hydrophilicity that provides a barrier to pathogen adherence and penetrance into the epithelium. Alteration in both secreted and membrane-associated mucins occur in drying ocular surface diseases. At the ocular surface, three types of mucins are present. The large gel-forming mucin MUC5AC is expressed by conjunctival goblet cells. Some cells of the lacrimal gland acini express the small soluble mucin MUC7. The corneal and conjunctival epithelia express the membrane-associated mucins MUCs 1, 4, and 16.
[Assay Principle]
Mucins are family of high molecular (1000 kda-10000 kda) and heavily glycosylated protein. Mucin domains within the protein core are rich in threonine and serine. The reducing ends of sugar chain N-acetylgalactosamin (GalNAc) are linked to those amino acids by the post-translational O-glicosylation. Mucin content can be measured as reducing ends of sugar chain afterβ-eliminated by diluted alkali. Reducing ends of sugar chain react at high temperatures with 2-cyanoacetamide (2-CAN) to produce intensely fluorescent condensate.
Tear Mucin Assay Kit (O-Glycan Assay Method)
Tear Mucin Assay Kit (O-Glycan Assay Method)
Tear Mucin Assay Kit (O-Glycan Assay Method)
Figure Explain:
IMAGE 1: Mucins expressed around corneal surface
IMAGE 2: Structure of mucin
IMAGE 3: Barrier function by tear mucins

Gipson IK1, Hori Y, Argüeso P. Character of ocular surface mucins and their alteration in dry eye disease. Ocul Surf. 2004 Apr;2(2):131-48.

Argüeso P1, Balaram M, Spurr-Michaud S, Keutmann HT, Dana MR, Gipson IKGipson IK et al., Decreased levels of the goblet cell mucin MUC5AC in tears of patients with Sjögren syndrome, Invest Ophthalmol Vis Sci, 2002

Uchino Y1, Uchino M2, Yokoi N3, Dogru M1, Kawashima M1, Okada N1, Inaba T4, Tamaki S4, Komuro A3, Sonomura Y3, Kato H3, Argüeso P5, Kinoshita S3, Tsubota, Alteration of tear mucin 5AC in office workers using visual display terminals: The Osaka Study. JAMA Ophthalmol. 2014 Aug;132(8):985-92. doi: 10.1001/jamaophthalmol.2014.1008.

Inatomi T1, Spurr-Michaud S, Tisdale AS, Zhan Q, Feldman ST, Gipson IK. Expression of secretory mucin genes by human conjunctival epithelia. Invest Ophthalmol Vis Sci. 1996 Jul;37(8):1684-92.

Susumu Honda, Yoshikazu Matsuda, Masaye Takahashi, and Kazuaki Kakehi, Fluorimetric Determination of Reducing Carbohydrates with2-Cyanoacetamide and Application to Automated Analysis of Carbohydrates as Borate Complexes. (1980) Analytical Chemistry, Vol.52, No. 7

Crowther RS, Wetmore RF: Fluorometric assay of O-linked glycoproteins by reaction with 2-cyanoacetamide. Anal Biochem 163: 170-174, 1987.