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Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)

Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)
Cat. No.
CSR-MBR-PMG-K01E
Quantity
100TEST
Price
¥ 105,600
$ 1408
€ 1056
Inventory
Available
Intended use
A complete reagent set to measure 2DG in cells after cell lysis by sonication
Composition
  • substrate buffer 3ml*3vial
  • 2DG6P Solution (1 mM)500 µL*1 tube
  • Sample Diluent Buffer Concentrate(100x) 5 ml* 1vial
  • Fluorescent Substrate 120 µL*1 vial
  • Enzyme Solution 270 µL*1 tube
(Materials required but not supplied.)
  • Ultrapure water (distilled water)
  • 2-deoxyglucose suggested product
  • Bovine Serum Albumin suggested product
  • 96-well black wall microplate
  • 96-well fluorescent plate reader
  • Suitable sonication device for cell lysis.
Other
[Background]
Insulin is a hormone that lowers the blood glucose level and is known to help glucose enter cells through an effect that enhances the activity of glucose transport, primarily in insulin-sensitive cells, such as adipocytes and skeletalmuscles.
The effect of insulin and other growth factors on cells is often assessed in the laboratory by measuring glucose uptake activity. Typically, glucose uptake is measured using 3-O-Methyl-D-Glucose or 2-deoxyglucose (2DG) labeled with radioactive 3H or other isotopes. However, the use of radioactive isotopes is not available to all labs and is subject to many restrictions. This assay kit was developed to provide a simple, rapid and convenient means to measure cellular glucose uptakewithout the use of radioisotope.
Storage
-20C
Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)
Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)
Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)
Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)
Figure Explain:
IMG1. Scheme of Assay Procedure
IMG2. Experimental Example Results: 2DG6P Calibration curve
IMG3. Experimental Example Results: Experimental Protocol and Result
IMG4. Products
Reference
  • Yamamoto N, et al ,(2006). A nonradioisotope, enzymatic assay for 2-deoxyglucose uptake in L6 skeletal muscle cells cultured in a 96-well microplate. Anal. Biochem. 351: 139-145.