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Products

Anti LC3 antibody

Anti LC3
Cat. No.
CAC-CTB-LC3-2-IC
Quantity
50UG
Price
¥ 60,000
$ 800
€ 600
Inventory
Available
Antigen/Source
LC3
Host
Mouse
Immunogen
Human
Reacts with
Human/Mouse
Clone
LC3-1703
Isotype
IgG1
Conjugation
Unlabeled
Fraction
Affinity Purified
Applications
Immunocytochemistry (cell)
Preservative
0.1% NaN3 in LIQ
Storage
-20C
Anti LC3
Anti LC3
Anti LC3
Anti LC3
Figure Explain:
IMG1: Immunofluorescence microscopy analysis of mouse embryonic fibroblasts (MEFs).
MEFs were cultured in regular DMEM supplemented with 10% FBS (left) or DMEM without amino acids (right) for 1 hr. After fixation with 4% paraformaldehyde for 10 min at room temperature, they were permeabilized with 50 µg/ml digitonin for 5 min. Cells were then subjected to immunofluorescence microscopy using #CAC-CTB-LC3-2-IC anti-LC3 antibody at 1:100 dilution. Scale bar, 20 µm.
IMG2: Immuno-electron microscopy analysis of mouse embryonic fibroblasts (MEFs).
MEFs were cultured in DMEM without amino acids for 2 hr. After fixation with 4% paraformaldehyde for 2 hr at room temperature, they were permeabilized with liquid nitrogen. Immuno-electron microscopy analysis (pre-embedding method) of endogenous LC3 was performed using #CAC-CTB-LC3-2-IC anti-LC3 antibody at 1:10 dilution. The gold labeling was intensified by using a silver enhancement kit (HQ silver enhancement kit, Nanoprobes, NY). Scale bar, 500 nm.
Reference
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Citation
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