Example of Western Blot, BAM-64-035-EX
Western Blot analysis of Tetanus Toxin with anti-Tetanus Toxin antibody (TH11)
Left Panel is SDS-PAGE analysis of partially purified tetanus toxin, silver stained, and Right panel is western blot analysis of the same samples. Lane 1 sample was reduced and Lane 2 sample was unreduced. Tetanus toxin Heavy and Light chains are so tightly attached and they are difficult to separate completely by heating in SDS in the presence of mercaptoethanol. In the above reduced samples, part of the reduced sample remained attached as shown by “H+L” in lane 1 of Left panel . To dissociate the two attached fragments completely, addition of 2M urea is required The antibody (TH11) recognizes Light chain of the toxin. The anti-tetanus toxin antibody was used at 1/1,000 dilution.