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Reagents for Genetic Engineerring

Taq DNA Polymerase(-dNTPs), with Robust buffer


Thermus aquaticus DNA polymerase (Taq DNA polymerase) was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa. This enzyme is suitable for PCR reactions; capable of amplifying DNA with various primers.


1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine) at the 3’-blunt ends for cloning into TA vector.

*Use of excess amount of the enzyme is not recommended.
General composition of PCR reaction mixture (total 50 µl)
Taq DNA polymerase (5 units/µl) 0.25 µl*
10 x Reaction Buffer (Taq) 5 µl
2.5mM (each) dNTPs 4 µl
Template < 500ng
Primer 1 0.2 ~ 1.0 M (final conc.)
Primer 2 0.2 ~ 1.0 M (final conc.)
Sterile distilled water up to 50 µl

< SDS-PAGE of Taq DNA polymerase >

< PCR products obtained by using Robust buffer (agarose gel electrophoresis) >

NOTE : Cautions for usage of Robust Buffer (Taq ) Robust Buffer induces maximum enzymatic activity. Therefore, cares should be taken to avoid production of undesirable smear bands in gel electrophoresis analysis by longer than optimal reaction time. We recommend about 5 to 10 seconds / kb elongation time for template up to 8 kb, and about 15 seconds / kb for up to 14 kb. We will recommend roughly the same elongation time to be set with 2-step PCR (shuttle PCR) and 3-step PCR. Extend the elongation time by short steps when amplification is not seen. The results of your experiments can be observed more rapidly by adopting 2-step PCR.

Product List

Product Name Cat# Quantity Price

Taq DNA polymerase Economy (-dNTPs) with Robust buffer

BAM-02-012-EX 200UNIT

¥ 3,000
$ 40
€ 30

Taq DNA polymerase Economy (-dNTPs) with Robust buffer

BAM-02-012-5EX 5*200UNIT

¥ 12,000
$ 160
€ 120

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.