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Reliable quality and great cost-performance!

S1 Nuclease

from Aspergillus sp.

Background

S1 Nuclease is a single-stranded-specific endonuclease that hydrolyzes single-stranded DNA and RNA into acid-soluble 5’- mononucleotides. It hydrolyzes single-stranded regions in duplex DNA such as loops and gaps.

Feature and Advantages

 

Fig.1 Thermal-stability and activity Stability:
30 minutes treatment Activity: pH4.6 
Fig.2 pH-stability and activity Stability:
2 hours treatment at 5°C Activity: 37°C

 

Specifications

Storage.....................................10mM sodium acetate, pH4.6
   150mM NaCl
   0.05M ZnSO4 5
   0% glycerol

Activity....................................≧100 units/μl
Molecular Weight........................32,000 glycoprotein containing Zn2+
Optimum pH and temperature..... See Fig. 1, 2 pH and
thermal stability ............ See Fig. 1, 2

ASSAY

 

Unit Definition
One unit of enzyme hydrolyzes 1μg of heat-denatured calf thymus DNA into acid-soluble form at 37°C, pH4.6 in one minute.


Composition of Supplied Reagents (Store at -20°C):
10×S1 Nuclease Buffer............300mM Sodium acetate, pH4.6
   2800mM NaCl
   10mM ZnSO4

Application Example

 

Selective degradation of single DNA
   DNA 1μg
   S1 Nuclease
   10U 10×S1
   Buffer 2μl dH2O up to 20μl
   =================
   Total volume 20μl

Procedure
After incubation for 15 minutes at 23°C, terminate by EDTA. Blunting of protruding termini with T4 DNA Polymerase or Klenow Fragment.

STORAGE:
-20°C

Product List

Product Name Cat# Quantity Price

S1 Nuclease

OZK-OZ-40-EX 200UL

¥ 10,000
$ 134
€ 100

References

> Lee, B. R., Kitamoto, K., Yamada, O. and Kumagai, C. (1995) Appl Microbial Biotechinol, 44, 425-431.
> Vogt, V.M. (1973) Eur. J. Biochem, 33, 192-200.
> Berk, A. J. and Sharp, P. A. (1978) Proc. Natl. Acad. Sci. USA, 75, 1274-1278.
> Wiegand, R. C., Godson, G. N. and Radding, C. M. (1975) J. Biol. Chem., 250, 8848-
8855.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.