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Rad51 Protein (human), functional

Background

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1, BRCA2, and P53 for the maintenance of genome stability (1). Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein (Fig. 1). Since the tag was removed from the recombinant protein (it still contains Gly-Ser-His at the N-terminal), it has been shown to retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52 (2). This product was confirmed to possess single strand DNA stimulated ATPase activity.

Application

1. Studies on homologous recombination in mammals including human
2. Studies on the interaction of Rad51 protein with various proteins
3. To be used as a standard for Western blotting

BAM_10001EX_1
Fig.1 Analysis of purified human Rad51 protein by SDS-PAGE

Product List

Product Name Cat# Quantity Price

Rad51 Protein

BAM-10-001-EX 20UG

¥ 42,000
$ 560
€ 420

Rad51 Protein

BAM-10-002-EX 100UG

¥ 126,000
$ 1680
€ 1260

References

1) Friedberg EC et al DNA Repair and Mutagenesis 2nd ed., ASM Press (2006)
2) Kurumizaka H et al “Human Rad51 amino acid residues required for Rad52 binding.” J Mol Biol 291:
537-548 (1999) PMID: 10448035
3) Murayama Y et al “Formation and branch migration of Holliday junctions mediated by eukaryotic
recombinases.” Nature 451:1018-1021 (2008) PMID: 18256600

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.