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Proliferating Cell Nuclear Antigen (PCNA), human, functional

Background

Proliferating cell nuclear antigen (PCNA) is a 36 kDa homotrimeric protein known to act as a co-factor for DNA polymerase δ, which is responsible for leading strand DNA replication. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle. A cell cycle-dependent protein called cyclin was shown to be identical to PCNA. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Human PCNA was over-expressed in E. coli as a recombinant full-size protein without any tag and highly purified.

Application

1. Research for DNA replication, recombination and repair.

2. Identification of proteins that interact with PCNA.

3. Useful for studying autoimmune diseases such as systemic lupus erythematosus.

Feature and Advantages

Highly purified: Greater than 98% purity determined by SDS-PAGE (CBB staining)

BAM_10152EX_1

Product List

Product Name Cat# Quantity Price

PCNA

BAM-10-151-EX 20UG

¥ 31,500
$ 420
€ 315

PCNA

BAM-10-152-EX 100UG

¥ 94,500
$ 1260
€ 945

References

1) Friedberg EC et al (2006) DNA repair and mutagenesis, 2nd Edition, ASM Press Washington,D.C.


2) Ohta S et al (2002) “A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding
proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel
PCNA-binding protein.” J Biol Chem 277: 40362-40367 PMID: 12171929


3) Iida T et al (2002) “PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated
DNA.” Genes Cells 7: 997-1007 PMID: 12354094


4) Fukuda K et al (1995) “Structure-Function Relationship of the Eukaryotic DNA Replication Factor,
Proliferating Cell Nuclear Antigen “J Biol Chem 270: 22527-22534 PMID: 12171929

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.