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useful for quantitative determination of human PYY in human serum and plasma sample.

New Anti Human PYY[peptide YY (PYY)] EIA Kit

   Back to "Quantitative determination kits for Biomarkers"

Background

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for human peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level in human blood decreases after resection of the intestine, possibly be due to the decrease in number of the endocrine cells secreting PYY. The EIA kit is prepared by using synthetic human PYY (3-36) as standard and biotinylated human PYY (3-36) as labeled antigen. The kit can be used for measurement of PYY [both PYY(3-36) and PYY(1-36)] in human serum or plasma with high sensitivity. It will be a specifically useful tool for PYY research.

HumanPYY: Y-P-I-K-P-E-A-P-G-E-D-A-S-P-E-E-L-N-R-Y-Y-A-S-L-R-H-Y-L-N-L-V-T-R-Q-R-Y-amide
RatPYY: Y-P-A-K-P-E-A-P-G-E-D-A-S-P-E-E-L-S- R-Y-Y-A-S-L-R-H-Y-L-N-L-V-T-R-Q-R-Y-amide

Features

This EIA kit is used for quantitative determination of human PYY [both PYY(3-36) and PYY(1-36)] in serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. Human PYY (3-36) standard is highly purified synthetic product.

Feature of new kit herePDF

・The assay kit can measure human PYY within the range of 0.082-20 ng/mL.
・The assay is completed within 16-18 hr.+3 hr.
・With one assay kit, 41 samples can be measured in duplicate.
・Test sample : Human serum and plasma Sample volume : 50 μL
・The 96-wells plate in kit was consisted by 8-wells strips, and the strips can be used separately.
・Precision and reproducibility
  Intra-assay CV(%)  Human serum 3.67-5.13  Human plasma 6.08-8.52
  Inter-assay CV(%)  Human serum 2.33-6.55  Human plasma 5.45-10.26
・Stability and Storage Store all of the components at 2-8°C.
・The kit is stable under the condition for 6 months from the date of manufacturing.
・The expiry date is stated on the package.

<Specificity>
The EIA kit shows 100% cross reactivity to human PYY (3-36) and human PYY (1-36), and shows less than 0.003% cross reactivity to human and rat NPY, which have similar amino acid sequence with human PYY

<Principle>
This EIA kit for determination of human PYY in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to human PYY and biotin-avidin affinity system. To the wells of plate coated with rabbit anti human PYY antibody, standard or samples, labeled antigen are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled streptoavidin-biotinylated antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of human PYY is calculated.

Product List

Product Name Cat# Quantity Price

PYY EIA

YII-YK080-EX 1KIT

¥ 76,000
$ 1014
€ 760

References
  • Adrian, T.E., Smith, H.A., Calvert, S.A., Aynsley-Green, A. and Bloom, S.R. (1986): Elevated plasma peptide YY in human neonates and infants.
    Pediatr Res.,20:1225-1227.
  • Adrian T.E., Ferri, G.L., Bacarese-Hamilton, A.J., Fuessl, H.S., Polak, J.M. and Bloom, S.R.(1995): Human distribution and release of a putative new gut hormone, peptide YY. Gastroenterology, 89: 1070-1077.
  • El-Salhy, M., Grimelius, L., Wilander, E., Ryberg B., Terenius, L., Lundburg, J.M. and Tatemoto, K.(1983): Immunocytochemical identification of polypeptide YY(PYY) cells in the human gastrointestinal tract.
    Histochemistry, 77:15-23.
  • Greeley, G.H.Jr., Hashimoto, T., Izukura, M., Gomez, G., Jeng, J., Hill, F.L., Liuis, F.,and Thompson, J.C.(1989): A comparison of intraduodenally and intracolonically administered nutrients on the release of peptide YY in the dog.
    Endocrinology, 125:1761-1765.
  • Greeley, G.H.Jr., Hill, F. L., Spannagel, A.and Thompson, J.C.(1987): Distribution of peptide YY in gastrointestinal tract of the rat, dog, and monkey.
    Regul.Pept., 19, 365-372.
  • Gomez, G., Zhang, T., Rajaraman, S., Thakore, K.N., Yanaihara, N., Townsend C.M.Jr., Thompson, J.C. and Greeley, G.H.Jr.(1995): Intestinal peptide YY : ontogeny of gene expression in rat bowel and trophic actions on rat and mouse bowel.
    Am J.Physiol. 268, G71-G81.
  • Larhammar, D.(1996): Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide:
    Regul.Pept., 62, 1-11.