This enzyme immunoassay (EIA) kit is a stable and convenient assay system for human peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level in human blood decreases after resection of the intestine, possibly be due to the decrease in number of the endocrine cells secreting PYY. The EIA kit is prepared by using synthetic human PYY (3-36) as standard and biotinylated human PYY (3-36) as labeled antigen. The kit can be used for measurement of PYY [both PYY(3-36) and PYY(1-36)] in human serum or plasma with high sensitivity. It will be a specifically useful tool for PYY research.
This EIA kit is used for quantitative determination of human PYY [both PYY(3-36) and PYY(1-36)] in serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. Human PYY (3-36) standard is highly purified synthetic product.
Feature of new kit here
・The assay kit can measure human PYY within the range of 0.082-20 ng/mL.
The EIA kit shows 100% cross reactivity to human PYY (3-36) and human PYY (1-36), and shows less than 0.003% cross reactivity to human and rat NPY, which have similar amino acid sequence with human PYY
This EIA kit for determination of human PYY in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to human PYY and biotin-avidin affinity system. To the wells of plate coated with rabbit anti human PYY antibody, standard or samples, labeled antigen are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled streptoavidin-biotinylated antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of human PYY is calculated.