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useful for quantitative determination of mouse GLP-2 in serum or plasma samples.

Mouse GLP-2 [Glucagon-Like Peptide-2] EIA Kit

Background

The proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produced the different proglucagon derived peptides( PGDPs ) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cell produces several structurally related peptides, including glucagon-like peptide(GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation.


Quantitative determination kits for Biomarker Flyer download [PDF]

Features

This EIA kit is used for quantitative determination of mouse GLP-2 in serum or plasma samples.
The kit is characterized for sensitive quantification, high specificity and no influence with other components in serum or plasma and needlessness of sample pre-treatment. Mouse GLP-2 standard is highly purified synthetic product.

  • The assay kit can measure mouse GLP-2 in the range of 0.412 - 100 ng/mL
  • The assay completes within 16-18 hr.+ 1.5 hr.
  • With one assay kit, 41 samples can be measured in duplicate
  • Test sample: mouse serum and plasma Sample volume 25 μL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility
        Intra-assay CV(%) Serum 4.9-8.8 Plusma 3.5-6.0
        Inter-assay CV(%) Serum 11.0-14.7 Plusma 4.9-16.0
  • Stability and Storage Store all of the components at 2-8°C. 12 months from the date of manufacturing. The expiry date is described on the label of kit.
  • <Specificity>
    The EIA kit has high specificity to mouse GLP-2 and shows no cross reactivity with mouse glucagon and mouse GLP-1 even in the concentration of 300 pmol/mL

    <Principle>
    This EIA kit for determination of mouse GLP-2 in serum or plasma samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat GLP-2 (strong cross reactivity to mouse GLP-2) and biotin-avidin affinity system. The 96-wells plate is coated with goat anti rabbit IgG antibody. Mouse GLP-2 standard or samples, labeled antigen and anti rat GLP-2 polyclonal antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated rat GLP-2- antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse GLP-2 is calculated.

    Composition

    Component Form Quantity Main Ingredient
    1. Antibody coated plate MTP*1 1 plate
    (96 wells)
    Goat anti rabbit IgG
    2. Mouse GLP-2 standard lyophilized 1 vial
    (50ng)
    Synthetic mouse GLP-2-
    3. Labeled antigen lyophilized 1 vial Biotinylated rat GLP-2
    4. Specific Antibody lliquid 1 bottle
    (6 mL)
    Rabbit anti rat GLP-2
    5. SA-HRP solution liquid 1 bottle
    (12 mL)
    HRP labeled streptoavidin
    6. Substrate buffer Liquid 1 bottle
    (26 mL)
    0.015% Hydrogen Peroxide
    7. OPD tablet tablet 2 tablets o-Phenylenediamine dihydrochloride
    8. Stopping solution liquid 1 bottle
    (12 mL)
    2N H2SO4
    9. Buffer solution Liquid 1 bottle
    (25 mL)
    Phosphate buffer
    10. Washing solution
    (concentrated)
    Liquid 1 bottle
    (50 mL)
    Concentrated saline
    11. Adhesive foil   3 sheets  

    MTP*1 ….. Microtitration plat

    Product List

    Product Name Cat# Quantity Price

    Glucagon-Like Peptide 2 EIA

    YII-YK142-EX 1KIT

    ¥ 76,000
    $ 1014
    € 760

    Glucagon EIA

    YII-YK090-EX 1KIT

    ¥ 76,000
    $ 1014
    € 760

    GlucagonLike Peptide 1 EIA KIT

    YII-YK160-EX 1KIT

    ¥ 76,000
    $ 1014
    € 760

    Glucagon-Like Peptide 2 EIA

    YII-YK140-EX 1KIT

    ¥ 76,000
    $ 1014
    € 760

    Glucagon-Like Peptide 2 EIA

    YII-YK141-EX 1KIT

    ¥ 76,000
    $ 1014
    € 760

    Glicentin, EIA

    YII-YK111-EX 1KIT

    ¥ 76,000
    $ 1014
    € 760

    Anti Total Glucagon

    YII-YP040-EX 50UL

    ¥ 40,000
    $ 534
    € 400

    Anti GLP 1 (7-36)amide

    YII-Y320-EX 50UL

    ¥ 35,000
    $ 467
    € 350

    Anti GLP 2 (14-33)

    YII-Y321-EX 50UL

    ¥ 35,000
    $ 467
    € 350

    Anti GLP 2

    YII-Y322-EX 50UL

    ¥ 35,000
    $ 467
    € 350

    Anti GLP-2

    YII-Y323-EX 50UL

    ¥ 35,000
    $ 467
    € 350

    Anti Glicentin (1-32)

    YII-Y324-EX 50UL

    ¥ 35,000
    $ 467
    € 350