The proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produced the different proglucagon derived peptides( PGDPs ) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cell produces several structurally related peptides, including glucagon-like peptide(GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation.
Quantitative determination kits for Biomarker Flyer download [PDF]
This EIA kit is used for quantitative determination of human GLP-2 in plasma samples. It has a lot of advantage to perform the assay, such as good quantification, high specificity and no influence with other body fluid factors or physiological active substances.
Human GLP-2 standard is highly purified synthetic product.
The EIA kit has high specificity to human GLP-2 and shows no cross reactivity with glucagon and GLP-1 within the range of 300 pmol/mL.
This EIA kit for determination of human GLP-2 in plasma samples is based on a competitive enzyme immunoassay using combination with highly specific antibody to rat GLP-2(using high cross reactivity between rat and human GLP-2) and biotin avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG antibody. Human GLP-2 standard or samples, biotinylated GLP-2 and anti rat GLP-2 polyclonal antibody are added to the wells for competitive immunoreaction. After rinsing out excess human GLP-2, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin biotinylated GLP-2 antibody complexs are formed on the surface of the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of human GLP-2 is calculated.
|1.||Antibody coated plate||Microtiter plate||1 plate
|Goat anti rabbit IgG antibody|
|2.||Human GLP-2 standard||Lyophilized||1 vial
|Synthetic human GLP-2|
|3.||Labeled antigen||Lyophilized||1 vial||Biotinylated human GLP-2|
|4.||GLP-2 antibody||Liquid||1 bottle
|Rabbit anti human GLP-2 antibody|
|5.||SA-HRP solution||Liquid||1 bottle
|HRP labeled SA|
|6.||Substrate buffer||Liquid||1 bottle
|Citrate buffer containing 0.015% hydrogen peroxide|
|7.||OPD tablet||Tablet||2 tablets||o-Phenylenediamine dihydrochloride|
|8.||Stopping solution||Liquid||1 bottle
|9.||Buffer solution||Liquid||1 bottle
|11.||Adhesive foil||3 pieces|
MTP*1 ….. Microtitration plat
Glucagon-Like Peptide 2 EIA