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useful for quantitative determination of human GLP-2 in plasma samples.

Human GLP-2 [Glucagon-Like Peptide-2] EIA Kit

Background

The proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produced the different proglucagon derived peptides( PGDPs ) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cell produces several structurally related peptides, including glucagon-like peptide(GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation.


Quantitative determination kits for Biomarker Flyer download [PDF]

Feature and Advantages

This EIA kit is used for quantitative determination of human GLP-2 in plasma samples. It has a lot of advantage to perform the assay, such as good quantification, high specificity and no influence with other body fluid factors or physiological active substances.
Human GLP-2 standard is highly purified synthetic product.

  • The assay kit can measure human GLP-2 in the range of 0.412-100 ng/mL
  • The assay completes within 16-18 hr.+ 1.5 hr.
  • With one assay kit, 41 samples can be measured in duplicate
  • Test sample: human serum and plasma Sample volume 25 μL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility Intra-assay CV(%) Serum 3.0-5.5 Plusma 3.7-4.8 Inter-assay CV(%) Serum 14.3-17.5 Plusma 13.0-16.4
  • Stability and Storage Store all of the components at 2-8°C. 9 months from the date of manufacturing. The expiry date is described on the label of kit.

<Specificity>
The EIA kit has high specificity to human GLP-2 and shows no cross reactivity with glucagon and GLP-1 within the range of 300 pmol/mL.

<Principle>
This EIA kit for determination of human GLP-2 in plasma samples is based on a competitive enzyme immunoassay using combination with highly specific antibody to rat GLP-2(using high cross reactivity between rat and human GLP-2) and biotin avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG antibody. Human GLP-2 standard or samples, biotinylated GLP-2 and anti rat GLP-2 polyclonal antibody are added to the wells for competitive immunoreaction. After rinsing out excess human GLP-2, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin biotinylated GLP-2 antibody complexs are formed on the surface of the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of human GLP-2 is calculated.

Composition

 ComponentFormQuantityMain Ingredient
1. Antibody coated plate Microtiter plate 1 plate
(96 wells)
Goat anti rabbit IgG antibody
2. Human GLP-2 standard Lyophilized 1 vial
(50ng)
Synthetic human GLP-2
3. Labeled antigen Lyophilized 1 vial Biotinylated human GLP-2
4. GLP-2 antibody Liquid 1 bottle
(6 mL)
Rabbit anti human GLP-2 antibody
5. SA-HRP solution Liquid 1 bottle
(12 mL)
HRP labeled SA
6. Substrate buffer Liquid 1 bottle
(26 mL)
Citrate buffer containing 0.015% hydrogen peroxide
7. OPD tablet Tablet 2 tablets o-Phenylenediamine dihydrochloride
8. Stopping solution Liquid 1 bottle
(12 mL)
1M H2SO4
9. Buffer solution Liquid 1 bottle
(25 mL)
Phosphate buffer
10. Washing solution
(concentrated)
Liquid 1 bottle
(50 mL)
Concentrated saline
11. Adhesive foil   3 pieces  

MTP*1 ….. Microtitration plat

Product List

Product Name Cat# Quantity Price

Glucagon-Like Peptide 2 EIA

YII-YK141-EX 1KIT

¥ 76,000
$ 1014
€ 760