The proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produced the different proglucagon derived peptides( PGDPs ) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cell produces several structurally related peptides, including glucagon-like peptide(GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation.
This EIA kit is used for quantitative determination of rat GLP-2 in serum or plasma samples. It has a lot of advantage to perform the assay, such as good quantification, high specificity and no influence with other body fluid factors or physiological active substances.
Rat GLP-2 standard is highly purified synthetic product.
The EIA kit has high specificity to rat GLP-2 and shows no cross reactivity with rat glucagon and rat GLP-1 within the range of 300 pmol/mL.
This EIA kit for determination of rat GLP-2 in serum or plasma samples is based on a competitive enzyme immunoassay using combination with highly specific antibody to rat GLP-2 and biotin avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG antibody. Rat GLP-2 standard or samples, biotinylated rat GLP-2 and anti rat GLP-2 polyclonal antibody are added to the wells for competitive immunoreaction. After rinsing out excess rat GLP-2, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin biotinylated rat GLP-2 antibody complexs are formed on the surface of the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of rat GLP-2 is calculated.
|1. Antibody coated plate||MTP*1||1 plate
|Goat anti rabbit IgG|
|2. Rat GLP-2 standard||lyophilized||1 vial
|Synthetic rat GLP-2|
|3. Labeled antigen||lyophilized||1 vial||Biotinylated rat GLP-2|
|4. Rat GLP-2 Antibody||lliquid||1 bottle
|Rabbit anti rat GLP-2|
|5. SA-HRP solution||liquid||1 bottle
|HRP labeled streptoavidin|
|6. Substrate buffer||liquid||1 bottle
|0.015% Hydrogen Peroxide|
|7. OPD tablet||tablet||2 tablets||o-Phenylenediamine dihydrochloride|
|8. Stopping solution||liquid||1 bottle
|9. Buffer solution||Liquid||1 bottle
|10. Washing solution
|11. Adhesive foil||3 sheets|
MTP*1 ….. Microtitration plat
Glucagon-Like Peptide 2 EIA