Glicentin is a 69-amino-acid peptide containing glucagon and oxyntomodulin sequences in the molecule. It is suggested that glicentin and oxyntomodulin are produced in the intestinal L-cells and glucagon in A-cells in the pancreas, these peptides are derived from a common precursor by two different tissue-specific processing pathways. In 1983, the amino acid sequence of human glicentin was deduced by Bell et al. from the genomic sequence of human preproglucagon. Glicentin is a major form of gut glucagon-like immunoreactants (Gut GLIs).
In mammalian small intestine, proglucagon is processed into glicentin, oxyntomodulin, and glucagon -like peptide 1(GLP-1) and glucagon -like peptide 2 (GLP-2). GLP-1(7-37) and GLP-1(7-36)amide have been isolated from the intestine and pancreas. It has been known that the GLP-1 sequence is well conserved between species in all mammals studied. Using synthetic peptides, several investigators have demonstrated that in contrast to GLP-1 (1-37), truncated GLP-1(7-36)amide and GLP-1(7-37) have several physiological effects. However, the physiological role of glicentin, a major gut glucagon, is still unclear. It has been known that the circulating level of plasma glicentin-like peptides increases significantly nutrient ingestion.
This EIA kit is used for quantitative determination of rat glicentin in plasma sample. The kit is characterized for sensitive quantification, high specificity and no influence with other components in plasma and needlessness of sample pre-treatment. Glicentin standard used in the kit is a highly purified synthetic product.
The EIA kit is specificity for rat glicentin. It does not exhibit cross-reactions with human glicentin, glucagon (rat, mouse and human), GLP-1 (rat, mouse & human) and rat GLP-2.
This EIA kit for determination of rat glicentin in plasma samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat glicentin and biotin-avidin affinity system. The 96-wells plate is coated with goat anti rabbit IgG antibody. Rat glicentin standard or samples, biotinylated rat glicentin and rabbit anti rat glicentin antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated rat glicentin-antibody complex on the surface on the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of rat glicentin is calculated.
|1. Antibody coated plate||MTP*1||1 plate (96 wells)||Goat anti rabbit IgG|
|2. Glicentin standard||lyophilized||1 vial (50pmol)||Synthetic rat glicentin|
|3. Labeled antigen||lyophilized||1 vial||Biotinylated rat glicentin|
|4. Glicentin antibody||lliquid||1 bottle (6 mL)||Rabbit anti rat glicentin|
|5. SA-HRP solution||liquid||1 bottle (12 mL)||HRP labeled streptoavidin|
|6. Substrate buffer||Liquid||1 bottle (26 mL)||0.015% Hydrogen Peroxide|
|7. OPD tablet||tablet||2 tablets||o-Phenylenediamine dihydrochloride|
|8. Stopping solution||liquid||1 bottle (12 mL)||1N H2SO4|
|9. Buffer solution||Liquid||1 bottle (10 mL)||Phosphate buffer|
|10. Washing solution (concentrated)||Liquid||1 bottle (50 mL)||Concentrated saline|
|11. Adhesive foil||3 sheets|
MTP*1 ….. Microtiter plate