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useful for quantitative determination of total mouse C-peptide, i.e. C-peptide I+II, in its serum and plasma samples.

Mouse C-Peptide EIA Kit

   Back to "Quantitative determination kits for Biomarkers"

Background

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide (mouse C-peptide I+II) in its serum and plasma.
The processing of proinsulin, which occurs within the pancreatic B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function.
This EIA kit includes synthetic mouse C-peptide II as standard antigen and biotinylated mouse C-peptide II as labeled antigen. The kit also contains specific polyclonal antibody recognizing mouse C-peptide I and mouse C-peptide II equivalently.
We have already developed EIA kits which can measure specifically mouse C-peptide I (YK011) and C-peptide II (YK012), respectively and now mouse C-peptide (I+II) kit (YK013) is developed for measuring C-peptide I and C-peptide II together. Namely this kit is useful for measuring total C-peptide level in mouse blood

Feature and Advantages

This EIA kit is used for quantitative determination of total mouse C-peptide, i.e. C-peptide I+II, in its serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse C-peptide II of this kit is a highly purified synthetic product (purity: higher than 98%).

  • This assay kit can measure mouse C-peptide (I+II) within the range of 0.412-100 ng/mL
  • The assay is completed within 18-20 hr + 1.5 hr.
  • With one assay kit, 41 samples can be measured in duplicate ・Test sample: mouse plasma and serum Sample volume: 25 μL
  • The 96-well plate of this kit consists of 128-wells strips, so that divided use by the strips is possible at user's option.
  • Precision and reproducibility Intra-assay CV(%): Serum 1.4-3.1 Inter-assay CV(%): Serum 4.2-8.1
  • Stability and Storage Store all of the components at 2-8°C. 6 months from the date of manufacturing. The expiry date is described on the label of kit.

<Specificity>
This EIA kit shows 93.9% crossreactivity to mouse C-peptide I, 100% to mouse C-peptide II, 22.9% to rat C-peptide and 48.2% to rat C-peptide II. It shows below 2% crossreactivity to mouse insulin and no crossreactivity to human, dog and pig C-peptide.

<Principle>
This EIA kit for determination of mouse C-peptide in serum and plasma is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-peptide (I+II) and biotin-avidin affinity system. To the wells of the plate coated with goat anti rabbit IgG, labeled antigen, standard antigen or samples and rabbit anti mouse C-peptide antibody are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA-biotinylated antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’5,5’-tetramethyl benzidine (TMB) and the concentration of mouse C-peptide is calculated.

Product List

Product Name Cat# Quantity Price

C-Peptide EIA

YII-YK013-EX 1KIT

¥ 76,000
$ 1014
€ 760

References
  • Wahren, J et al: C-peptide makes a comeback. Diabetes Metab Res Rev, 19, 345-347, 2003
  • Pierson, CR et al: Proinsulin C-peptide replacement in type1 diabetic BB/Wor-rats prevents deficits in nerve fiber regeneration.
    J Neuropathol Exp Neurol, 62, 765-779, 2003
  • Li, ZG et al: C-peptide enhances insulin-mediated cell growth and protection against high glucose-induced apoptosis in SH-SY5Y cells.
    Diabetes Metab Res Rev, 19, 375-385, 2003
  • Johansson, J et al: Molecular effects of proinsulin C-peptide
    Biochem Biophy Res Comm, 295, 1035-1040, 2002
  • Lindon, H et al: C-peptide exerts cardioprotective effects in myocardical ischemia-reperfusion
    Am J Phsiol Heart Circ Physiol, 279, 1453-1459, 2000
  • Matsuda, K and Oka, Y: C-peptide (CPR). Nippon Rinsho Vol 57, 313-316, 1999
  • Wentworth, BM et al: Characterization of the two nonallelic genes encoding mouse preproinsulin
    J Mol Evol, 23, 305-312, 1986