This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide II in its plasma, serum and urine. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. The EIA kit is prepared by using synthetic mouse C-Peptide II as standard and biotinylated mouse C-Peptide II as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of mouse C-Peptide II. We have already developed mouse C-peptide I EIA kit and mouse C-peptide I+II kit is also been developing in our laboratory.
This ELISA kit is used for quantitative determination of mouse C-Peptide II in its plasma, serum & urine samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples. Mouse C-Peptide II standard is a highly purified synthetic product (purity: higher than 98%).
The EIA kit shows following cross reactivity of 0.091% to mouse C-Peptide I, 0.34% to rat C-Peptide I, 63.3% to rat C-Peptide II and 1.8% to rat insulin and no cross reactivity to human and dog C-Peptide.
This EIA kit for determination of mouse C-Peptide II in serum, plasma and urine samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-Peptide II and biotin-avidin affinity system. The 96 wells plate is coated with goat anti-rabbit IgG. C-Peptide II standard or samples, labeled antigen and rabbit anti mouse C-Peptide II antibody are added to the wells for competitive immunoreaction. After incubation and plate wash, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated mouse C-Peptide II-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse C-Peptide II is calculated.
C-Peptide II, ELISA