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useful for quantitative determination of mouse C-Peptide II in its plasma, serum & urine samples

Mouse C-Peptide II EIA Kit

   Back to "Quantitative determination kits for Biomarkers"

Background

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide II in its plasma, serum and urine. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. The EIA kit is prepared by using synthetic mouse C-Peptide II as standard and biotinylated mouse C-Peptide II as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of mouse C-Peptide II. We have already developed mouse C-peptide I EIA kit and mouse C-peptide I+II kit is also been developing in our laboratory.

Characteristics

This ELISA kit is used for quantitative determination of mouse C-Peptide II in its plasma, serum & urine samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples. Mouse C-Peptide II standard is a highly purified synthetic product (purity: higher than 98%).

<Specificity>
The EIA kit shows following cross reactivity of 0.091% to mouse C-Peptide I, 0.34% to rat C-Peptide I, 63.3% to rat C-Peptide II and 1.8% to rat insulin and no cross reactivity to human and dog C-Peptide.

<Test Principle>
This EIA kit for determination of mouse C-Peptide II in serum, plasma and urine samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-Peptide II and biotin-avidin affinity system. The 96 wells plate is coated with goat anti-rabbit IgG. C-Peptide II standard or samples, labeled antigen and rabbit anti mouse C-Peptide II antibody are added to the wells for competitive immunoreaction. After incubation and plate wash, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated mouse C-Peptide II-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse C-Peptide II is calculated.

Product List

Product Name Cat# Quantity Price

C-Peptide II, ELISA

YII-YK012-EX 1KIT

¥ 76,000
$ 1014
€ 760

References
  • Wahren, J. et al: C-peptide makes a comeback.
    Diabetes Metab. Res. Rev., 19 (5),345-347, 2003
  • Pierson, CR. et al: ProinsulinC-peptide replacement in type1diabetic BB/Wor-rats prevents deficits in nerve fiber regeneration.
    J. Neuropathol. Exp. Neurol., 62 (7), 765-79, 2003
  • Li, ZG. et al: C-peptide enhances insulin-mediated cell growth and protection against high glucose-induced apoptosis in SH-SY5Y cells.
    Diabetes Metab. Res. Rev., 19 (5), 375-85, 2003
  • Johansson, J. et al: Molecular efffects of proinsulin C-peptide.
    Biochem. Biophy. Res.Comm., 295, 1035-1040, 2002
  • Lindon, H. et al: C-peptide exerts cardioprotective effects in myocardical ischemia-reperfusion.
    Am. J. Phsiol. Heart Circ. Physiol., 279 (4), 1453-1459, 2000
  • Matsuda M. and Oka, Y: C-peptide (CPR), Nippon Rinsho Vol 57, 1999Suppl, Wide range blood࡮urine chemical detection.
    Immunological Examination(4), 313-316, 1999
  • Wentworth, BM. et al: Characterization of the two nonallelic genes encoding mouse preproinsulin.
    J. Mol. Evol., 23 (4), 305-312, 1986
  • Yanaihara, C. et al: Immunoreactive rat C-peptide I and II in glucose perfusate of isolated pancreas.
    In: Insulin (ed. Brandenburg, D., and Wollmer. A.), Walter de Gruyter &Co., Berlin, 651-657, 1980
  • Yanaihara, C. et al: Immunological studies on synthetic rat and guinea pig C-peptide.
    In: Proinsulin, Insulin, C-peptide (ed.Baba,S., Kaneko,S., and Yanaihara, N.), Excerpta Medica, Amsterdam-Oxford, 87-93, 1979