This enzyme immunoassay (EIA) kit is a stable and convenient assay system for rat C-peptide in its plasma, serum, urine and culture supernatant The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide and insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. The EIA kit is using synthetic rat C-Peptide I as standard and biotinylated rat C-Peptide I as labeled antigen. The kit contains specific polyclonal antibody (C-peptide antibody) recognized to the amino acid sequence in the C-terminal side region which are common between rat C-Peptide I and II.
This ELISA kit is used for quantitative determination of rat C-Peptide in its plasma, serum, urine and culture supernatant samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples. Rat C-Peptide standard is highly purified synthetic product (purity: higher than 98%).
The EIA kit has high specificity to rat C-Peptide and shows no cross reactivity to human and other animal species.
This EIA kit for determination of rat C-Peptide in plasma, serum, urine and culture supernatant samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat C-Peptide and biotin- avidin affinity system. The 96-wells plate is coated with goat anti rabbit IgG and C-Peptide standard or samples, labeled antigen and anti rat C-Peptide antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) be added to form HRP labeled streptoavidin-biotinylated rat C-Peptide-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of rat C-Peptide is calculated.
|1. Antibody coated plate||MTP*1||1 plate
|Goat anti rabbit IgG|
|2. C-Peptide standard||lyophilized||1 vial
|Synthetic mouse C-Peptide I|
|3. Labeled antigen||lyophilized||1 vial
|Biotinylated mouse C-Peptide I|
|4. C-Peptide antibody||liquid||1 bottle
|Rabbit anti mouse C-Peptide I|
|5. SA-HRP solution||liquid||1 bottle
|HRP labeled streptoavidin|
|6. Substrate buffer||liquid||1 bottle
|0.015% Hydrogen Peroxide|
|7. OPD tablet||tablet||2 tablets||o-Phenylenediamine dihydrochloride|
|8. Stopping solution||liquid||1 bottle
|9. Buffer solution
|10. Washing solution
|11. Adhesive foil||3 sheets|
MTP*1........ Microtitration plat