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useful for quantitative determination of mouse C-Peptide in its plasma, serum & urine samples.

Mouse C - Peptide I EIA Kit

   Back to "Quantitative determination kits for Biomarkers"

Introduction

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for rat C-peptide in its plasma, serum, urine and culture supernatant The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide and insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. The EIA kit is using synthetic rat C-Peptide I as standard and biotinylated rat C-Peptide I as labeled antigen. The kit contains specific polyclonal antibody (C-peptide antibody) recognized to the amino acid sequence in the C-terminal side region which are common between rat C-Peptide I and II.

Characteristics

This ELISA kit is used for quantitative determination of rat C-Peptide in its plasma, serum, urine and culture supernatant samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples. Rat C-Peptide standard is highly purified synthetic product (purity: higher than 98%).

<Specificity>
The EIA kit has high specificity to rat C-Peptide and shows no cross reactivity to human and other animal species.

<Test Principle>
This EIA kit for determination of rat C-Peptide in plasma, serum, urine and culture supernatant samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat C-Peptide and biotin- avidin affinity system. The 96-wells plate is coated with goat anti rabbit IgG and C-Peptide standard or samples, labeled antigen and anti rat C-Peptide antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) be added to form HRP labeled streptoavidin-biotinylated rat C-Peptide-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of rat C-Peptide is calculated.

Composition

ComponentFormQuantityMain Ingredient
1. Antibody coated plate MTP*1 1 plate
(96 wells)
Goat anti rabbit IgG
2. C-Peptide standard lyophilized 1 vial
(50ng)
Synthetic mouse C-Peptide I
3. Labeled  antigen lyophilized 1 vial
(1.5 ng)
Biotinylated mouse C-Peptide I
4. C-Peptide antibody liquid 1 bottle
(6 mL)
Rabbit anti mouse C-Peptide I
5. SA-HRP solution liquid 1 bottle
(12 mL)
HRP labeled streptoavidin
6. Substrate buffer liquid 1 bottle
(26 mL)
0.015% Hydrogen Peroxide
7. OPD tablet tablet 2 tablets o-Phenylenediamine dihydrochloride
8. Stopping solution liquid 1 bottle
(12 mL)
1M H2SO4
9. Buffer solution
(concentrated)
Liquid 1 bottle
(20 mL)
Phosphate buffer
10. Washing solution
(concentrated)
Liquid 1 bottle
(50 mL)
Concentrated saline
11. Adhesive foil   3 sheets  

MTP*1........ Microtitration plat

Product List

Product Name Cat# Quantity Price

C-Peptide EIA

YII-YK010-EX 1KIT

¥ 76,000
$ 1014
€ 760

References
  • Markussen, J. and Sundby, F. (1972): Eur. J. Biochem., 25: 153.
  • Massey, D. E. and Smyth, D. G. (1975): J. Biol. Chem. 250: 6288.
  • Miyachi, Y. Vaitukais, J. L. Nieschlag, E. and Lipsett, M. B. (1972): J. Clin. Endocr.,34: 23.
  • Smyth, D. G., Markussen, J. and Sundby, F. (1974): Nature (Lond), 248:151.
  • Tager, H. S., Emdin, S.O., Clark, J. L. and Steiner, D. F. (1973): J. 248: 3476
  • Tager, H. S. and Steiner, D. F. (1972): J. Biol. Chem., 247, 7936
  • Yanaihara, N., Sakagami, M., Sakura, N., Iizuka, Y., Nishida, T., Hashimoto, T. and Yanaihara, C.
    (1977): In: Deabetes. p116 Ed J.S.Bajaj. Excerpta Media, Amsterdam.
  • Yanaihara, N., Nishida, T., Sakagami, M., and Yanaihara, C.
    (1977): In: Peptide Chemistry 1976, p85 Ed T. Nakajima. Protein Research Foundation, Osaka.
  • Yanaihara, N., Yanaihara, C., Sakagami, Sakura, N., Hashimoto, T. and Nishida, T.
    (1978): Diabetes, 27, (Suppl 1 ) 149
  • Yanaihara, C., Ozaki, J., Nishida, T , Iizuka, Y., Sato H., Yanaihara, N., and Kaneko, T.
    (1979): p87.Eds. S. Baba, T. Kaneko, N. Yanaihara, Excerpta Medica, Amsterdam-Oxford
  • Luo, W. Q., Kanno, T., Winarto, A, Iwanaga, T., Li., J., Futai, Y. Yanaihara, C., and Yanaihara, N.
    (1998): Biomed, Res., 19, 127