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useful for quantitative determination of human PYY in human plasma sample.

Anti Human PYY[peptide YY] EIA Kit

Background

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for human peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level in human blood decreases after resection of the intestine, possibly be due to the decrease in number of the endocrine cells secreting PYY. The EIA kit is prepared by using synthetic human PYY as standard and biotinylated human PYY as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence in the central portion of human PYY.

HumanPYY: Y-P- I-K-P-E-A-P-G-E-D-A-S-P-E-E-L-N-R-Y-Y-A-S-L-R-H-Y-L-N-L-V-T-R-Q-R-Y-amide
RatPYY: Y-P-A-K-P-E-A-P-G-E-D-A-S-P-E-E-L-S- R-Y-Y-A-S-L-R-H-Y-L-N-L-V-T-R-Q-R-Y-amide

Feature and Advantages

This EIA kit is used for quantitative determination of human PYY in human plasma sample. The kit is characterized for sensitive quantification, high specificity and no influence with other components in plasma and needlessness of sample pre-treatment. PYY standard is highly purified synthetic product (purity: higher than 98%).

  • The assay kit can measure Human PYY in the range of 0.14-100 ng/mL
  • The assay completes within 16-20 hr.+ 2.5 hr.
  • With one assay kit, 40 samples can be measured in duplicate
  • Test sample: human plasma Sample volume 25 μL
  • The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
  • Precision and reproducibility
     Intra-assay CV(%) 11.23-14.52
     Inter-assay CV(%) 11.92-15.261
  • Stability and Storage Store all of the components at 2-8°C.
  • 6 months from the date of manufacturing. The expiry date is described on the label of kit.

<Specificity>
The EIA kit is specificity for human PYY, shows 1% cross reactivity to rat PYY and less than 0.01% cross reactivity to human and rat NPY, which have similar amino acid sequence with human PYY.

<Test Principle>
This EIA kit for determination of human PYY in plasma sample is based on the competitive enzyme immunoassay using combination of highly specific antibody to human PYY and biotin-avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG. PYY standard or samples, labeled antigen and anti human PYY antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin- biotinylated human PYY-antibody complex on the surface on the wells. Finally, HRP enzyme activity is determined by o-phenylenediamine dihydrochloride (OPD) and the concentration of human PYY is calculated.

Composition

ComponentFormQuantityMain Ingredient
1. Antibody coated plate MTP*1 1 plate(96 wells) anti rabbit IgG
2. Human PYY Standard lyophilized 1 vial (100ng) Synthetic human PYY
3. Labeled antigen lyophilized 1 vial(1.5 ng) Biotinylated rat PYY
4. PYY antibody lyophilized 1 bottle Rabbit anti Human PYY antibody
5. SA-HRP solution liquid 1 bottle (12 mL) HRP labeled streptoavidin
6. Substrate buffer liquid 1 bottle (25 mL) Citrate buffer containing 0.015% hydrogen eroxide
7. OPD tablet tablet 2 tablets o-Phenylenediamine dihydrochloride
8. Stopping solution liquid 1 bottle (12 mL) 1M H2SO4
9. Buffer solution
(concentrated)
Liquid 1 bottle (12 mL) Phosphate buffer
10. Washing solution
(concentrated)
Liquid 1 bottle (50 mL) Concentrated saline
11. Adhesive foil   3 sheets  

MTP*1 ... Microtitration plat

Product List

Product Name Cat# Quantity Price

PYY EIA

YII-YK080-EX 1KIT

¥ 76,000
$ 1014
€ 760

PYY EIA

YII-YK081-EX 1KIT

¥ 76,000
$ 1014
€ 760

Anti PYY

YII-Y070-EX 50UL

¥ 35,000
$ 467
€ 350

Anti PYY

YII-Y072-EX 50UL

¥ 35,000
$ 467
€ 350

Anti Pancreastatin (33-51)

YII-Y090-EX 50UL

¥ 35,000
$ 467
€ 350