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useful for quantitative determination of mouse leptin in mouse serum samples

Mouse Leptin ELISA Kit

   Back to "Quantitative determination kits for Biomarkers"

Background

Leptin, which is a product of ob gene, is a protein consisting of 146 amino acids and it is secreted from white adipose tissue. It is known that leptin acts on hypothalamus to decrease food intake and reduce body weight, body fat, blood sugar and blood insulin in a healthy and an ob/ob mice. Further, gene expression of neuropeptide Y (NPY) is suppressed by this protein. Recently, radioimmunoassay for leptin determination in human plasma has become available and leptin level in human patient group with obesity was found to increase in comparison with that in normal group. The plasma level well correlated with body fat and these observations showed clearly that leptin concentration in human plasma reflects tissue fat weight. The measurement of leptin in plasma or serum may be a good index of obesity. Although mouse leptin shows a high homology (96%) with rat leptin in amino acid sequences, it is observed that substitution of quite a number of residues occurs between human and mouse leptin. These findings have urged us to develop highly sensitive immunoassay system specific to rat or mouse leptin. Yanaihara Institute Inc. already has rat leptin ELISA kit (YK050 and YK051), and now we have developed mouse leptin ELISA kit for measuring mouse leptin in its serum. It will be a specifically useful tool for leptin researches.

Feature and Advantages

This ELISA kit is used for quantitative determination of mouse leptin in its serum samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse leptin, in this kit is a recombinant product.

  • This assay kit can measure mouse leptin within the range of 0.313-20 ng/mL
  • The assay is completed within 6.5 hr.
  • With one assay kit, 40 samples can be measured in duplicate
  • Test sample: serum Sample volume: 25 μL
  • The 96-well plate of this kit consists of 128-wells strips, so that divided use by the strips is possible at user’s option.
  • Precision and reproducibility
    Intra-assay CV(%) mouse serum 5.01-9.84
    Inter-assay CV(%) mouse serum 4.37-7.71
  • Stability and storage  Store all the components at 2-8°C. The kit is stable under the condition 6 months from the date of manufacturing

<Specificity>
This ELISA kit is highly specific to mouse leptin. It shows 33.8 % crossreactivity to rat leptin and no crossreactivity to human leptin.

<Test Principle>
This ELISA kit for determination of mouse leptin is based on a sandwich enzyme immunoassay. To the wells of plate coated with highly purified antibody against mouse leptin, standard antigen or sample is added for the 1st step immunoreaction. After the 1st step incubation and plate washing, biotinylated rabbit anti mouse leptin antibody is added as the 2nd step reaction to form leptin antibody- antigen -biotinylated leptin antibody complex on the surface of the wells. After the 2nd step incubation and rinsing out excess biotinylated antibody, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added for binding to biotinylated leptin antibody. Finally, HRP enzyme activity is determined by 3,3’,5,5’-tetramethylbenzidine (TMB) and the concentration of mouse leptin is calculated.

Product List

Product Name Cat# Quantity Price

Leptin, ELISA Kit

YII-YK052-EX 1KIT

¥ 60,000
$ 800
€ 600

References
  • Zhang, Y et al: Positional cloning of the mouse obese gene and its human homologue.
    Nature 372, 425, 1994
  • Pelleymounter, MA et al: Effects of the obese gene and product on body weight regulation in ob/ob mice.
    Science 299, 540, 1995
  • Funahashi, T et al: Enhanced expression of rat obese(ob) gene in adipose tissues of ventromedial hypothalamus(VMH)-lesioned rats.
    Biochem Biophys Res Commun 211, 469, 1995
  • McGregor, G et al: Radioimmunological measurement of leptin in plasma of obese and diabetis human subject.
    Endocrinology 137, 1501, 1996
  • Sainsburry, A et al: Intracerebroventricular administration of neuropeptide Y to normal rats increase obese gene expression in white adipose tissues.
    Diabetologia 39, 353, 1996
  • Hosoda, H et al: Development of radioimmunoassay for human leptin.
    Biochem Biophys Res Commun 221, 234, 1996
  • Hashimoto, H et al: Parathyroid hormone-related protein induces cachectic syndromes without directly modulating the expression of hypothalamic feeding-regulating peptides.
    Clin Cancer Res 13, 292, 2007