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for quantitative determination of insulin in plasma sample

Insulin ELISA Kit and related product

   Back to "Quantitative determination kits for Biomarkers"

Background

This kit is a stable and convenient assay system for measurement of insulin (human, rabbit and dog) in serum samples. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide. Insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of insulin in blood is very important, and also provides valuable information to evaluate the pancreatic B cell function.
This kit for determination of insulin concentration (IR-insulin) in serum samples of human, rabbit and dog is based on a sandwich enzyme immunoassay by using combination of guinea pig anti human insulin antibody (coated on plate), recombinant insulin standard, biotinylated guinea pig anti human insulin antibody and HRP labeled streptoavidin (SA-HRP). Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.

Features

This ELISA kit is used for quantitative determination of insulin in plasma sample of human, rabbit and dog. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples and needlessness of sample pretreatment. Human insulin standard is recombinant product.

<Specificity>

This kit shows the following crossreactivities: 100% to human insulin, rabbit insulin and dog insulin, and 20% crossreactivity to human proinsulin.

<Test Principle>

This ELISA kit for determination of insulin (IR-insulin) in serum is based on a sandwich enzyme immunoassay. The 96-wells plate is coated with guinea pig anti human insulin antibody and insulin standard or samples are added to the wells for their immunoreaction. After incubation and plate washing, biotinylated guinea pig anti human insulin antibody is introduced to the wells and the antibody - antigen - labeled antibody complex is formed on the surface of the well. After rinsing out excessive labeled antibody, HRP labeled streptoavidin (SA-HRP) are added to bind to labeled antibody. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.

Component

    Form Quantity Main Ingredient
1. Antibody coated plate MTP*1 1plate(96 wells) 1 plate (96 wells) Guinea pig anti human insulin antibody
2. Standard Lyophilized 1 vial (100ng) Recombinant human insulin
3. Labeled antibody Liquid 1 bottle (12 mL) Biotinylated guinea pig anti human insulin
4. SA-HRP solution Liquid 1 bottle (12 mL) HRP labeled streptoavidin
5. Substrate buffere Liquid 1 bottle (24 mL) 0.015% hydrogen peroxide
6. OPD tablet dihydrochloride Liquid 2 tablets o-Phenylenediamine
7. Stopping solution Liquid 1 bottle (12 mL) 1M H2SO4
8. Buffer solution Liquid 1 bottle (20 mL) Phosphate buffer
9. Washing solution
(concentrated)
Liquid 1 bottle (50 mL) っcConcentrated saline
10. Adhesive foil   3 sheets  

MTP*1.....Microtitration plate

Product List

Product Name Cat# Quantity Price

Insulin ELISA

YII-YK060-EX 1KIT

¥ 50,000
$ 667
€ 500

Anti Insulin

YII-Y370-EX 50UL

¥ 35,000
$ 467
€ 350

References
  • Bell GI, Pictet RL et al., Sequence of the human insulin gene. Nature 284: 26-32, 1980
  • Y. Zhang et al., Ionic mechanisms underlying abnormal QT prolongation and the associated arrhythmias in diabetic rabbits :
    A role of rapid delayed rectifier K+ current. Cell Physiol Biochem 19: 225-238, 2007