This kit is a stable and convenient assay system for measurement of insulin (human, rabbit and dog) in serum samples. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide. Insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of insulin in blood is very important, and also provides valuable information to evaluate the pancreatic B cell function.
This kit for determination of insulin concentration (IR-insulin) in serum samples of human, rabbit and dog is based on a sandwich enzyme immunoassay by using combination of guinea pig anti human insulin antibody (coated on plate), recombinant insulin standard, biotinylated guinea pig anti human insulin antibody and HRP labeled streptoavidin (SA-HRP). Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.
This ELISA kit is used for quantitative determination of insulin in plasma sample of human, rabbit and dog. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples and needlessness of sample pretreatment. Human insulin standard is recombinant product.
This kit shows the following crossreactivities: 100% to human insulin, rabbit insulin and dog insulin, and 20% crossreactivity to human proinsulin.
This ELISA kit for determination of insulin (IR-insulin) in serum is based on a sandwich enzyme immunoassay. The 96-wells plate is coated with guinea pig anti human insulin antibody and insulin standard or samples are added to the wells for their immunoreaction. After incubation and plate washing, biotinylated guinea pig anti human insulin antibody is introduced to the wells and the antibody - antigen - labeled antibody complex is formed on the surface of the well. After rinsing out excessive labeled antibody, HRP labeled streptoavidin (SA-HRP) are added to bind to labeled antibody. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.
|1.||Antibody coated plate||MTP*1||1plate(96 wells)||1 plate (96 wells) Guinea pig anti human insulin antibody|
|2.||Standard||Lyophilized||1 vial (100ng)||Recombinant human insulin|
|3.||Labeled antibody||Liquid||1 bottle (12 mL)||Biotinylated guinea pig anti human insulin|
|4.||SA-HRP solution||Liquid||1 bottle (12 mL)||HRP labeled streptoavidin|
|5.||Substrate buffere||Liquid||1 bottle (24 mL)||0.015% hydrogen peroxide|
|6.||OPD tablet dihydrochloride||Liquid||2 tablets||o-Phenylenediamine|
|7.||Stopping solution||Liquid||1 bottle (12 mL)||1M H2SO4|
|8.||Buffer solution||Liquid||1 bottle (20 mL)||Phosphate buffer|
|Liquid||1 bottle (50 mL)||っcConcentrated saline|
|10.||Adhesive foil||3 sheets|