S-100 protein has a molecular weight of 21K Dalton and is consist of two subunits, α chain and β chain. It is known that combination of these subunits is different from the location in human body. S-100ββ is localized in glial cell and schwann cell, S-100αβ in glial cell and S-100αα in striated muscle, heart and kidney.
It was reported that the concentration of S-100β in cerebrospinal fluid was an useful marker for diagnosis of the degree of brain damage after head injury, cerebral hemorrhage and ischemic stroke. Recently another report described that the increasing of S-100β in blood correlated to the degree of brain damage after cerebral ischemia, infarction, hemorrhage and severe head injury.
Quantitative determination kits for Biomarker Flyer download [PDF]
This ELISA kit is used for quantitative determination of S-100β in plasma sample. The kit is characterized by sensitive quantification and high specificity. In addition, it is not influenced by other components in plasma sample and needlessness of sample pre-treatment.
The ELISA kit shows 0.2% cross reactivity to bovine S-100αα.
This kit for determination of S-100β in plasma sample is based on the sandwich enzyme immunoassay. During first immune incubation, S-100β in standards or in samples bind to the rabbit anti bovine S-100β antibody, which is coated on the surface of the microtiter plate. After incubation and plate washing, labeled antibody solution (biotinylated rabbit anti bovine S-100β polyclonal antibody) is added to bind to the antibody-antigen complex. Then, HRP labeled streptoavidin (SA-HRP) is added to form antibody-antigen-biotinylated antibody complex. Finally, HRP enzyme activity is determined by o-phenylenediamine dihydrochloride (OPD) and the concentration of S-100β is calculated.
|1.||Antibody coated plate||MTP*1||1plate(96 wells)||Rabbit anti bovine S-100β|
|2.||S-100ß Standard||Lyophilized||1 vial (5ng)||Bovine S-100ß|
|3.||Labeled antibody||Liquid||1 bottle (11 mL)||Biotinylated Rabbit Anti bovine S-100ß|
|4.||SA-HRP solution||Liquid||1 bottle (11 mL)||HRP labeled streptoavidin|
|5.||Substrate buffere||Liquid||1 bottle (26 mL)||Citrate buffer containing 0.015% Hydrogen Peroxide|
|6.||OPD tablet||Tablet||2 tablets||o-Phenylenediamine hydrochloride|
|7.||Stopping solution||Liquid||1 bottle (11 mL)||1M H2SO4|
|8.||Buffer solution||Liquid||1 bottle (20 mL)||Phosphate buffer|
|Liquid||1 bottle (50 mL)||Concentrated saline|
|10.||Adhesive foil||4 pieces|