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for quantitative determination of rat C-Peptide in its plasma, serum & urine samples.

Rat C-Peptide EIA Kit and related products


This enzyme immunoassay (EIA) kit is a stable and convenient assay system for rat C-peptide in its plasma, serum and urine. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide and insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. The EIA kit is prepared by using synthetic rat C-Peptide I as standard antigen and biotinylated rat C-Peptide I as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence in the C-terminal side region which are common between rat C-Peptide I and II. This ELISA kit is used for quantitative determination of rat C-Peptide in its plasma, serum & urine samples. It has a lot of advantage to perform the assay, such as good quantification, no influence with other body fluid factors or physiogical active substances and needlessness of sample pretreatment. Rat C-Peptide standard is highly purified synthetic product ( purity: higher than 98% ) and biotinylated peptide is stable because N biotinylglycylglycyl Rat C-Peptide I is used for it.

Quantitative determination kits for Biomarker Flyer download [PDF]


Specificity The EIA kit has high specificity to rat C-Peptide and shows no cross reactivity to human and other animal species. Test Principle This EIA kit for determination of rat C-Peptide in plasma and urine samples is based on a competitive enzyme immunoassay using combination with highly specific antibody to rat C-Peptide and biotin - avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG and C-Peptide standard or samples, biotinylated rat C-Peptide and anti rat C-Peptide antibody are added to the wells for competitive immunoreaction. After rinsing out excess rat C-Peptide, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin - biotinylated rat C-Peptide - antibody complexs are formed on the surface on the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of rat C-Peptide is calculated.


  Form Quantity Main Ingredient
1. Antibody coated plate MTP*1 1plate(96 wells) Anti rabbit IgG
2. C-Peptide standard lyophilized 1 vial Synthetic rat C-Peptide I( 50ng)
3. Labeled antigen lyophilized 1 vial Biotinylated rat C-Peptide I
4. C-Peptide antibody liquid 1 bottle (12 mL) Rabbit anti rat C-Peptide
5. SA-HRP solution liquid 1 bottle (12 mL) HRP labeled streptoavidin
6. Substrate buffer liquid 1 bottle (24 mL) 0.015% Hydrogen Peroxide
7. OPD tablet tablet 2 tablets o-Phenylenediamine hydrochloride
8. Stopping solution liquid 1 bottle (12 mL) 2N-H2SO4
9. Buffer solution liquid 1 bottle (35 mL) Phosphate buffer
10. Washing solution liquid 1 bottle (50 mL) Concentrated saline
11. Adhesive foil   3 sheets  

MTP*1 ..... Microtiration plate

Product List

Product Name Cat# Quantity

C-Peptide EIA