This enzyme immunoassay (EIA) kit is a stable and convenient assay system for rat C-peptide in its plasma, serum and urine. The processing of proinsulin, which occurs within the B cell, yields insulin and C-peptide and insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. The EIA kit is prepared by using synthetic rat C-Peptide I as standard antigen and biotinylated rat C-Peptide I as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence in the C-terminal side region which are common between rat C-Peptide I and II. This ELISA kit is used for quantitative determination of rat C-Peptide in its plasma, serum & urine samples. It has a lot of advantage to perform the assay, such as good quantification, no influence with other body fluid factors or physiogical active substances and needlessness of sample pretreatment. Rat C-Peptide standard is highly purified synthetic product ( purity: higher than 98% ) and biotinylated peptide is stable because N biotinylglycylglycyl Rat C-Peptide I is used for it.
Quantitative determination kits for Biomarker Flyer download [PDF]
Specificity The EIA kit has high specificity to rat C-Peptide and shows no cross reactivity to human and other animal species. Test Principle This EIA kit for determination of rat C-Peptide in plasma and urine samples is based on a competitive enzyme immunoassay using combination with highly specific antibody to rat C-Peptide and biotin - avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG and C-Peptide standard or samples, biotinylated rat C-Peptide and anti rat C-Peptide antibody are added to the wells for competitive immunoreaction. After rinsing out excess rat C-Peptide, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin - biotinylated rat C-Peptide - antibody complexs are formed on the surface on the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of rat C-Peptide is calculated.
|1.||Antibody coated plate||MTP*1||1plate(96 wells)||Anti rabbit IgG|
|2.||C-Peptide standard||lyophilized||1 vial||Synthetic rat C-Peptide I( 50ng)|
|3.||Labeled antigen||lyophilized||1 vial||Biotinylated rat C-Peptide I|
|4.||C-Peptide antibody||liquid||1 bottle (12 mL)||Rabbit anti rat C-Peptide|
|5.||SA-HRP solution||liquid||1 bottle (12 mL)||HRP labeled streptoavidin|
|6.||Substrate buffer||liquid||1 bottle (24 mL)||0.015% Hydrogen Peroxide|
|7.||OPD tablet||tablet||2 tablets||o-Phenylenediamine hydrochloride|
|8.||Stopping solution||liquid||1 bottle (12 mL)||2N-H2SO4|
|9.||Buffer solution||liquid||1 bottle (35 mL)||Phosphate buffer|
|10.||Washing solution||liquid||1 bottle (50 mL)||Concentrated saline
|11.||Adhesive foil||3 sheets|
MTP*1 ..... Microtiration plate