Urocortin 2 (Ucn2), also known as stresscopin-related peptide, is a novel predicted neuropeptide related to corticotropin-releasing factor (CRF). The peptide consisting of 38 amino acid residues was first demonstrated to be expressed centrally and to bind selectively to type 2 CRF receptor (CRFR2). In the rodent, Ucn2 transcripts were shown to be expressed in the discrete regions of the central nervous system including stress-related cell groups in the hypothalamus and brainstem. More recently, the expression of Ucn2 transcripts was detected in the olfactory bulb, pituitary, cortex, hypothalamus, and spinal cord. Ucn2 mRNA was also found to be expressed widely in a variety of peripheral tissues, most highly in the skin and skeletal muscle tissues. Ucn2-like immunoreactivity was detected by RIA in acid extracts of mouse brain, muscle, and skin. Immunohistochemically Ucn2 was found in both skin epidermis and adnexal structures and in the skeletal muscle myocytes. Ucn2 gene transcription was stimulated in the hypothalamus and brainstem by glucocorticoid administration to the mouse and inhibited by removal of glucocorticoids by adrenalectomy, suggesting a putative link between the CRFR1 and CRFR2 pathways. On the other hand, in the rat a stressor-specific regulation of Ucn2 mRNA expression in the hypothalamic paraventricular nucleus was demonstrated, which raised the possibility of a modulary role of Ucn2 mRNA in stress-induced alteration of anterior and posterior pituitary function, depending on the type of stress. Administration of dexamethasone to the mouse resulted in a decrease of Ucn2 mRNA levels in the back skin region. Adrenalectomy signifcantly increased Ucn2 mRNA levels in the skin, and the levels were reduced back to normal levels after corticoid replacement. We succeeded this time in the development of mouse urocortin 2 EIA kit which highly specific for mouse Ucn2 with almost no crossreaction to Ucn1 (mouse, rat), Ucn3 (mouse), ACTH (mouse, rat) and CRF (mouse, rat, human). The kit can be used for measurement of Ucn2 in mouse plasma or serum with high sensitivity. It will be a specifically useful tool for Ucn2 research.
The assay kit can measure mouse urocortin 2 in the range of 0.82-200 ng/mL.
The assay completes within 16-18 hr.+3 hr.
With one assay kit, 41 samples can be measured in duplicate.
Test sample: Mouse plasma & serum
Sample volume: 20 L
The 96-well plate in kit was consisted by 8-wells strips. The kit can be used separately.
Precision and reproducibility
Mouse plasma & serum
Stability and Storage
Store all of the components at 2-8?.
6 months from the date of manufacturing.
The expiry date is described on the label of kit.
The EIA kit has high specificity to mouse urocortin 2 and shows cross reactivity neither urocortin 1 (mouse, rat), urocortin 3 (mouse), ACTH (mouse, rat) nor CRF (mouse, rat, human).
|1.||Antibody coated plate||MTP*1||1 plate (96 wells)||Rabbit anti mouse urocortin 2 antibody|
|2.||Standard||lyophilized||1 vial||Synthetic mouse urocortin 2 (200ng/vial)|
|3.||Labeled antigen||lyophilized||1 vial||Biotinylated mouse urocortin 2|
|4.||SA-HRP solution||liquid||1 bottle (12 mL)||HRP labeled streptoavidin|
|5.||Substrate buffer||liquid||1 bottle (24 mL)||0.015% Hydrogen peroxide|
|6.||OPD tablet||tablet||2 tablets||o-Phenylenediamine dihydrochloride|
|7.||Stopping solution||liquid||1 bottle (12 mL)||1 M H2SO4|
|8.||Buffer solution||liquid||1 bottle (15 mL)||Phosphate buffer|
|9.||Washing solution||liquid||1 bottle (50 mL)||Concentrated saline
|10.||Adhesive foil||3 sheets|
MTP*1 ….. Microtiter plate
Urocortin 2, EIA
Urocortin 3, EIA Kit
Anti Urocortin (3-40)
Anti Urocortin 1
Anti Urocortin 2
Anti Urocortin 3