GLP-1 is a peptide hormone from the intestinal mucosa, which is produced from its precursor, proglucagon by post transnational processing. The mammalian proglucagon is synthesized in the neuroendocrine L-cell of the intestine and the alpha-cells of the pancreas. It contains within its structure the sequences of glucagon and two glucagon-like peptides (GLP-1 and GLP-2) in tandem flanked at their amino and carboxyl termini by dibasic residues. GLP-1 is a 37 amino acids peptide and produced in the small intestine and in the pancreas in the human, in either C-terminal-amidated on glycine-extended form. GLP1 (7-36) amide and its receptor are present in several brain regions and may play a role in the physiological control of feeding. Several reports have been presented as follows as to the biological activities of GLP-1. GLP-1 (7-37) and (7-36) amide is known as one of the most potent insulin secretagogues.
Yanaihara Institute Inc. developed a quantitative EIA kit with high specificity and sensitivity (detection limit 0.206ng/mL) for rat/mouse/human GLP-1 (YK160) as a useful tool for these necessaries.
Quantitative determination kits for Biomarker Flyer download [PDF]
The EIA kit has high specificity to rat/mouse/human GLP-1 and shows cross reactivity neither rat/human/mouse glucagons, human glicentin nor rat/mouse/human GLP-2.
Rat plasma 89.20-110.4%, Human plasma 86.0-101.7%
|1.||Antibody coated plate||MTP*1||1 plate (96 wells)||Goat anti rabbit IgG|
|lyophilized||1 vial||Synthetic GLP-1 (7-36) amide|
|3.||Labeled antigen||lyophilized||1 vial||Biotinylated GLP-1 (7-36) amide|
|4.||GLP-1 antibody||liquid||1 bottle (6 mL)||Rabbit anti-GLP-1 (7-36) amide|
|5.||SA-HRP||liquid||1 tube (0.2 mL)||HRP labeled streptoavidin|
|6.||Diluent for SA-HRP||liquid||1 bottle (12 mL)||Phosphate buffer|
|7.||Substrate buffer||liquid||1 bottle (26 mL)||0.015% Hydrogen Peroxide|
|8.||OPD tablet||tablet||2 tablets||o-Phenylenediamine dihydrochloride|
|9.||Stopping solution||liquid||1 bottle (10 mL)||2N H2SO4|
|10.||Buffer solution||liquid||1 bottle (10 mL)||Phosphate buffer|
|Liquid||1 bottle (50 mL)||Concentrated saline|
|12.||Adhesive foil||3 sheets|
MTP*1 ….. Microtiter plate
GlucagonLike Peptide 1 EIA KIT