For determination of Rat, Mouse or Human Glucagon
According to many studies on glucagon immunoassay, it has been established that the antibody against the C - terminal fragment ( 19 - 29 ) of glucagon has specific binding with pancreatic glucagon, whereas the antibody against the N - terminal fragment ( 9 - 19 ) of glucagon has specific binding with both of pancreatic and intestinal glucagon ( total glucagon ). Once, 30K by Unger et. al had been widely used as an antibody specific for the C - terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic glucagon specific antibody using synthetic peptide with the C - terminal fragment ( 19 - 29 ) of glucagon as immunogen in 1981.
This EIA kit has been developed by using polyclonal antibody against glucagon ( 19 - 29 ), synthetic glucagon as standard antigen and biotinylated glucagon as labeled antigen for the measurment of rat, mouse or human glucagon in plasma
|Storage||Store at 2 to 8 °C|
|Shelf life||12 month|
|Assay range||50 - 10,000 pg/mL|
|For 41 test samples in duplicate|
|Test sample||Plasma sample|
|Test sample volume||100L(50L)|
|Test duration||20 24 hours+1.5hours(100L)
44 48 hours+1.5hours(50L)
Biomarker mesurement kit Flyer download [PDF]
This EIA kit is used for quantitative determination of rat, mouse or human pancreatic glucagon in plasma sample. It has a lot of advantage to perform the assay, such as good quantification, high specificity and no influence with other body fluid factors or physiogical active substances.
Glucagon standard is highly purified synthetic product ( purity: higher than 98% ) and biotinylated peptide is purified by HPLC.
The EIA kit has high specificity to pancreatic glucagon and shows no cross reactivity with intestinal glucagon, GLP-1 and GLP-2.
This EIA kit for determination of rat, mouse or human pancreatic glucagon in plasma sample is based on a competitive enzyme immunoassay using combination with highly specific antibody to glucagon and biotin avidin affinity system. The 96 wells plate is coated with rabbit anti glucagon and glucagon standard or samples, and biotinylated glucagon are added to the wells for competitive immunoreaction. After rinsing out excess rat, mouse or human glucagon, HRP labeled streptoavidins are added to bind to the antigen-antibody complex so that HRP labeled streptoavidin - biotinylated glucagon antibody complexs are formed on the surface of the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of rat, mouse or human pancreatic glucagon is calculated.
|Rabbit anti glucagon|
|2.||Glucagon standard||lyophilized||1 vial||Synthetic glucagon(10ng/vial)|
|3.||Labeled antigen||lyophilized||1 vial||Biotinylated pancreatic glucagon|
|4.||Specific antibody||liquid||1 bottle
|HRP labeled streptoavidin|
|5.||Substrate buffer||liquid||1 bottle
|0.015% Hydrogen Peroxide|
|6.||OPD tablet||tablet||2 tablets||o-Phenylenediamine hydrochloride|
|7.||Stopping solution||liquid||1 bottle
|8.||Buffer solution(A)||Liquid||1 bottle
|Phosphate buffer including serum|
|9.||Buffer solution(B)||Liquid||1 bottle
|11.||Adhesive foil||4 sheets|
MTP*1 ….. Microtitration plat