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Essential for citrullinated protein research

Anti PAD2(Peptidylarginine Deiminase 2) polyclonal antibody

Background

Peptidylarginine deiminases (PADs) are a group of post-translational modification enzymes that citrullinate (deiminate) protein arginine residues in a calcium ion-dependent manner, yielding citrulline residues. In mammalian tissues, PADs are found as five different isoforms (PAD1, PAD2, PAD3, PAD4/5 and PAD6). Among them, only PAD2 (76-kDa protein) is a proven occupant of the central nervous system. Immunocytochemical studies have localized PAD2 in glial cells, especially astrocytes, microglial cells and oligodendrocytes. Also PAD2 expressed in multiple tissues including the epidermis, skeletal muscle, kidney, lung, spleen, pancreas, stomach, intestine, ovary, uterus, testis and leukocyte. Citrullinated proteins are known to be involved in the onset of various diseases such as articular rheumatism, Alzheimer's disease, multiple sclerosis and psoriasis.

Immunohistochemical staining


Hippocampus of Alzheimer’s Disease stained with PAD2 antibody (ROI002) at 1:300 dilution and developed by 3,3’-diaminobenzidine. Dentate gyrus and stratum radiatum of CA1 and CA2 areas stained.

Western Blot Analysis

Lane : Human brain tissue extract
PAD2 antibody (ROI002) at 1:1,000 dilution used.[Data from Dr. Akihito Ishigami, Tokyo Metropolitan Institute of Gerontology]

 

<Reference>
1. Ishigami, A. et al., Abnormal accumulation of citrullinated proteins catalyzed by peptidylarginine deiminase in hippocampal extracts from patients with Alzheimer’s disease. J. Neurosci. Res. 80 120-128 (2005)
2. Ishigami, A. et al., Human peptidylarginine deiminase type II: Molecular cloning, gene organization and expression in human skin. Arch. Biochem. Biophys. 407 25-31 (2002)

Product List

Product Name Cat# Quantity Price

Anti PAD2

SML-ROI002-EX 0.1ML

¥ 30,000
$ 400
€ 300

Anti SMP30 [Regucalcin, Gluconolactonase (GNL)]

SML-ROI001-EX 0.1ML

¥ 30,000
$ 400
€ 300

Anti GFAP

SML-ROI003-EX 0.2ML

¥ 30,000
$ 400
€ 300