c-Myc is a very strong proto-oncogene and it is very often found to be upregulated in many types of cancers. Through its role as a transcription factor, c-Myc alters the expression of hundreds of target genes, many of which are themselves oncogenes or tumor suppressors. 9E10 recognises recombinant proteins incorporating a c-Myc epitope tag and detects human c-Myc protein as well as a peptide in random coil configuration not as a helix.
|Specificity||c-Myc epitope tag|
|Antigen||Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa)
corresponding to 408-439 of Human c-Myc conjugated to KLH.
|Applications||ChIP, ELISA, IHC, IP, WB|
Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0)
Protein was expressed in E.coli and lysed (Crude sample).
Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting.
Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL)
Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution
Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag
Based on CBB Staining result, c-Myc tagged fluorescent protein had higher molecular weight
compared to fluorescent protein only (no tag). Based on Western Blotting result, c-Myc
tagged fluorescent protein was detected, though no band was detected with no tag.
Anti c-Myc (9E10)