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Anti c-Myc (9E10) antibody

Background

c-Myc is a very strong proto-oncogene and it is very often found to be upregulated in many types of cancers. Through its role as a transcription factor, c-Myc alters the expression of hundreds of target genes, many of which are themselves oncogenes or tumor suppressors. 9E10 recognises recombinant proteins incorporating a c-Myc epitope tag and detects human c-Myc protein as well as a peptide in random coil configuration not as a helix.

Specification

Host Mouse
Specificity c-Myc epitope tag
Antigen Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa)
corresponding to 408-439 of Human c-Myc conjugated to KLH.
Clone 9E10
Subclass IgG1
Cross reactivity Human
Applications ChIP, ELISA, IHC, IP, WB

Example Assay Data

Sample Preparation

Inserted c-Myc tag to C-terminus end of DasherGFP plasmid vector (DNA2.0)
Protein was expressed in E.coli and lysed (Crude sample).
Crude sample as separated by SDS-PAGE. Part of the fraction was used for CBB Staining, rest for Western Blotting.

Western Blotting

Primary Antibody (anti c-Myc [CAC-XIM-MA001]) 1 : 1000 dilution (1μg/mL)
Secondary Antibody (anti mouse IgG-HRP, Bethyl Laboratories, Cat.No. A90-216P) 1 : 3000 dilution



Anti c-Myc [CAC-XIM-MA001] detection of c-Myc tag
Based on CBB Staining result, c-Myc tagged fluorescent protein had higher molecular weight
compared to fluorescent protein only (no tag). Based on Western Blotting result, c-Myc
tagged fluorescent protein was detected, though no band was detected with no tag.

Product List

Product Name Cat# Quantity Price

Anti c-Myc (9E10)

CAC-XIM-MA001 200UG

¥ 49,000
$ 654
€ 490

References
  • Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960. (WB)
  • Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842. (ChIP)
  • Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916. (IP, WB)
  • Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822. (IP, WB)
  • Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423. (IHC)
  • Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455. (IHC)
  • Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969. (IP)
  • Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782. (WB)