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Anti mouse G9a / Ehmt2 mouse monoclonal antibody

G9a : Euchromatic histone-lysine N-methyltransferase 2

Background

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis.

Application

<Recommended Concentration>
In order to obtain the best results, optimal working dilutions should be determined by each individual user.
  Western Blot : 0.5 ug/mL
  ELISA : 0.1ug/mL
  Immunoprecipitation : Decide by use
  Supershift Assay : Not tested
  Chromatin immunoprecipitation : Not tested
  Immunohistochemistry : 5 ug/mL


WT mouse ES cell (Positive)

G9a KO mouse ES cell (Negative)

<Reference>
1. Tachibana M. et al., Genes Dev, 1; 19(7): 815-26, 2005.

Specificity

This antibody specifically recognizes mouse G9a and cross reacts with human G9a. Not yet tested in other species.

Product List

Product Name Cat# Quantity Price

Anti G9a/Ehmt2

PPX-PP-A8620A-00 0.1ML

¥ 50,000
$ 667
€ 500

Anti GLP/Ehmt1

PPX-PP-B0422-00 0.1ML

¥ 50,000
$ 667
€ 500