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To detect Salmonella in food

NH IMMUNOCHROMATO SALMONELLA

Background

Salmonella food poisoning is one of the most well known food poisoning in the world. In recent years, Salmonella has been one of the leading causes of food poisoning in Japan: a total of 144 food poisonings affected 3,700 people, resulting in one death in 2005.
Salmonella has more than 2,500 serotypes1), about 1,500 of which are pathogenic to humans and animals. Among them, S.Enteritidis has been rapidly emerging as a cause of food poisoning through chicken eggs and their products: food poisoning by S.Enteritidis accounts for 50% to 62% of all salmonella food poisonings2).
This kit is designed to detect S.Enteritidis, the most problematic bacterium among salmonella in food and provide test results in a short time with only a simple method due to its immuno-chromatographic principle

Application

1) Detection of Salmonella (S.Enteritidis) in food products
Note: This kit is intended to specifically detect S.Enteritidis and therefore cannot detect other serotypes of Salmonella.

Features

1) This product does not need skill because it can be handled only with a one-step procedure.
2) It rapidly provides a test result.
3) It needs no specific detector.

1)   Sensitivity test
When a sample containing S.Enteritidis ATCC 13076 is tested according to the "Preparation of sample solution" and "Test procedure" of the present instruction manual, a positive test result is obtained when the bacterial concentration of the sample is 1 ´ 106 CFU/mL or higher.

2)   Reproducibility test
When S.Enteritidis-positive and -negative sample solutions are simultaneously tested three times each, the positive solution is determined as positive and the negative solution as negative for all the repetitions.

3)   Minimum detection sensitivity
It was confirmed from a test using two standard and three isolated strains of S.Enteritidis that the minimum detection sensitivity of the kit ranged from 1 ´ 105 to 1 ´ 107 CFU/mL.

Note 1:   The minimum detection sensitivity of the kit may vary depending on the strains of S.Enteritidis, type of the enrichment culture medium, and sample components.

4)   Cross-reactivity
(1) No cross-reactivity has been detected with the following strains

 Control number Result
Escherichia coli ATCC 1175 -
Citrobacter freundii ATCC 8090, 43864 -
Enterobacter aerogenes ATCC 13048 -
Enterobacter cloacae ATCC 13047, 49141 -
Enterobacter sakazakii ATCC 51329 -
Klebsiella oxytoca ATCC 8724 -
Klebsiella pneumoniae ATCC 4352 -
Serratia liquefaciens ATCC 27592 -
Serratia marcescens ATCC 8100 -
Serratia odorifera ATCC 33077 -

(2) Among serotypes other than S.Enteritidis, this kit has been confirmed to respond to S. abaetetuba and S. anatum

Components of the Kit

1)  Components
A: Test plate: 5 tests × 4
B:  Instruction manual

2)  Components and volume (per test)
(1) Anti-Salmonella polyclonal antibody (rabbit)    0.25 g
(2) Gold colloid labeled anti-Salmonella polyclonal antibody (rabbit)    0.075 g
(3) Anti-rabbit immunoglobulin polyclonal antibody (goat)    0.25 g

Illustration of Test plate and the Principle of assay

1) Part names

a.  Sample dropping part (Take care not to touch it with your hands)
b.  Reagent containing part
c.  Spread area (Take care not to touch it with your hands or damage it)
d.  Absorption pad
e.  Measurement item entering area
f.  Test line appearance position (about 30 mm from the sample dropping part)
g.  Control line appearance position (about 38 mm from the sample dropping part)

2) Detection mechanism

When a sample solution is dropped at the sample dropping part of the test plate, the gold colloid labeled anti-Salmonella antibody (2) is dissolved to form complexes with Salmonella (1) in the sample solution.  The complexes migrate across the spread area by capillarity and are then trapped by the anti-Salmonella antibody (3) fixed at the test line appearance position to develop a reddish purple line by the gold colloid.  Thus, whether the sample solution contains Salmonella or not can be determined by visually inspecting the test line appearance position.
Irrespective of the presence/absence of Salmonella in the sample solution, the excess gold colloid labeled antibody further migrates across the spread area and is then trapped by the anti-rabbit immunoglobulin goat antibody (4) fixed at the control line appearance position to develop a reddish purple line.  It can be confirmed that the sample solution has migrated across the spread area by the development of the line.

Preparation of the sample Solution.......... refer for dtailsPDF

Operating Procedures for Testing.......... refer for dtailsPDF

Product List

Product Name Cat# Quantity Price

NH Immunochromato Salmonella

NPH-999300000 4*5TEST

¥ 10,000
$ 134
€ 100

References
  • Popff, M.Y.: Antigenic formulas of the Salmonella serovars, 8th ed, WHO Collaborating Centre for Reference and Research on Salmonella, Institute Pasteur (2001).
  • National Institute of Infectious Diseases: Pathogenic microbe detection information, Vol. 27, No. 8, 2006.
  • Japanese Ministry of Health, Labour, and Welfare: Guidelines on Food Hygiene Testing, Volume of Microorganisms, Japan Food Hygiene Association, 180-191 (2004).

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.