Immunohistochemistry, western blotting and ELISA
< Specificity >
Specific for free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. Cross reactivity have been tested for following analogues: Nitrotyrosine, chlorotyrosine, aminotyrosine, phosphotyrosine, tyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA)
< Applicable species >
This antibody can be applied to tissue samples from human, rabbit, rat and other animals, because lipid oxidation is species independent.
But if you are planning to apply to mouse tissue, blocking of internal mouse IgG will be needed before staining. For example, Vector M.O.M. Immunodetection kit (code.PK2200) may be useful for blocking of mouse IgG.
< Immunohistochemistry >
DT staining of mouse skin (Dr. Sugiyama, Tottori Univ.)