Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.
|Antigen:||MDA-modified keyhole-lympet hemocyanine.|
|Form:||Frozen (100 micro g/mL antibody in 10mM PBS containing 0.1 %NaN3 and 0.5% BSA). Purified by Protein-A.|
Recommended antibody concentration is 0.5-1.0 micro g/mL on paraformaldehyde fixed tissue.
|Specificity:||Specific for MDA-modified protein (especially DHP-lysine).|
|Storage:||Less than -20°C|
Specifity of anti-MDA antibody.
|Immunohistochemical detection of
MDA-modified protein in atherosclerotic aorta.
Dr. N Shibata,
Tokyo Women's Medical University
*This paper is a good example for immunohistochemical detection of MDA-modified protein and western blot analysis of oxidised LDL.