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HEL may be a useful biomarker for initial stage of lipid peroxidation

Hexanoyl-Lys (HEL) ELISA Kit and related product

   Oxidative stress marker product TOP

What is HEXANOYL-LYSINE ?

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

HEL ELISA kit is a competitive enzyme-linked immunosorbent assay for quantitative measurement of hexanoyl-Lys adduct. This kit is based on the monoclonal antibody clone 5H4, which is specific for HEL. Suitable for urine, serum and other biological samples. For research use only. Not for diagnostic nor medical use. Read entire insert before use.

Specifications

Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific to Hexanoyl-Lysine.
Measuring range: 2 - 700 nmol/L
Time to answer: ver night and 2 hours.
Format: 96 wells (54 samples in single assay)
Applications: Urine and serum samples from human and animals.
Storage: Store at 2 - 8?<C (don't freeze).
Expiry: 12 months after the day of manufacturing.
Required but not provided: 50 micro L micropipettor and pipette tips
8-channel (50-200 micro L) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 degree C incubator
Microtiter plate reader (measuring wavelength 450 nm)

Content of this kit

Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific to Hexanoyl-Lysine.
Measuring range: 2 - 700 nmol/L
Time to answer: ver night and 2 hours.
Format: 96 wells (54 samples in single assay)
Applications: Urine and serum samples from human and animals.
Storage: Store at 2 - 8?<C (don't freeze).
Expiry: 12 months after the day of manufacturing.
Required but not provided: 50 micro L micropipettor and pipette tips
8-channel (50-200 micro L) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 degree C incubator
Microtiter plate reader (measuring wavelength 450 nm)

Oxidative Stress Markers flyer download [PDF]

Product List

Product Name Cat# Quantity Price

Hexanoyl-Lys(HEL) ELISA kit

NNS-KHL-700E-EX 96WELL

¥ 76,800
$ 1024
€ 768

References
  • Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa.
    Formation of NȜ-(hexanonyl) lysine in protein exposed to lipid hydroperoxide.
    Journal of Biological Chemistry, Vol. 274, No. 29, pp. 20406 – 20414, 1999
  • Yoji Kato, Yoshiaki Miyake, Kanefumi Yamamoto, Yoshiharu Shimomura, Hirotomo Ochi, Yoko Mori, Toshihiko Osawa.
    Preparation of a monoclonal antibody to NȜ-(hexanonyl) lysine: applycation to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem.
    Biophys. Res. Commun., Vol. 274(2), pp389-393, 2000