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useful to a general oxidative stress marker in the body.

Highly Sensitive 8-OHdG Check ELISA KIT

8-OHdG top

8-hydroxy-2'-deoxyguanosine (8-OHdG / 8-oxo-dG) ELISA kit
Lower concentration of 8-OHdG can be measured with this kit.

8-OHdG Check"is a competitive in vitro enzyme-linked immunosorbent assay for quantitative measurement of the oxidative DNA adducts 8-hydroxy-2'-deoxyguanosine (8-OHdG) in various biological samples such as DNA of tissue, serum, urine etc., using anti-8-OHdG monoclonal antibody.

Differences between "New 8-OHdG Check" and "Highly Sensitive 8-OHdG Check"

Products  New 8-OHdG Check (KOG-200SE) Highly Sensitive 8-OHdG Check(KOG-HS10E)
Measurement Range 0.5-200 ng / ml 0.125-10 ng / ml
Primary Antibody Reaction 37°C, 1 hour 4°C, over night
Secondary Antibody Reaction 37°C, 1 hour room temperature, 1 hour
Application Research Fields
1. Development of treatment for diseases
2. Assessment of anti-oxidative function of food materials
3. Prevention of aging and diseases
4. Evaluation of life style
5. Evaluation of oxidative stress generated by toxic compounds, radiation, excess smoking, excess drinking, pharmaceutical dose of medicines, and so on
Kit Contents
1. Microtiter plate coated with 8-OHdG (split type)
2. Primary antibody (Anti-8-OHdG monoclonal antibody)
3. Primary antibody solution
4. Secondary antibody (POD-conjugated anti mouse antibody)
5. Secondary antibody solution
6. Chromatic solution (3,3',5,5'-tetramethylbenzidine)
7. Substrate solution
8. Washing solution
9. Reaction terminating solution
10. Standard 8-OHdG solution (0.125, 0.25, 0.5, 1, 4, 10 ng/ml)
11. Plate seal

Product List

Product Name Cat# Quantity Price

Highly Sensitive 8-OHdG Check ELISA kit

NNS-KOG-HS10E-EX 96WELL

¥ 102,400
$ 1366
€ 1024

References
  •  Cutler RG, Antioxidants and aging. Am J Clin Nutr. 1991 Jan;53(1 Suppl):373S-379S. PMID: 1985414
  •  Kasai H. et al, Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis. 1986 Nov;7(11):1849-51. PMID: 3769133
  •  Fraga CG. et al, Oxidative damage to DNA during aging: 8-hydroxy-2'-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4533-7. PMID: 2352934
  •  Shigenaga MK. et al, Assays for 8-hydroxy-2'-deoxyguanosine: a biomarker of in vivo oxidative DNA damage. Free Radic Biol Med. 1991;10(3-4):211-6. PMID: 1650737
  •  S.Okamoto, and H.Ochi Chemical Abst.129859a (1992)
  •  Kantha SS. et al, Carnosine sustains the retention of cell morphology in continuous fibroblast culture subjected to nutritional insult. Biochem Biophys Res Commun. 1996 Jun 14;223(2):278-82. PMID: 8670272
  •  Toyokuni S. et al, Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest. 1997 Mar;76(3):365-74. PMID: 9121119
  •  Erhola M. et al, Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2'-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett. 1997 Jun 9;409(2):287-91. PMID: 9202163
  •  H.Ochi "Rouka seigyo syokuhin no kaihatsu (Development of foods for controlling aging)" Korin Press, pp294 (1995)