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For assessment of oxidative stress in serum, tissue and cultured cells

New 8-OHdG Check ELISA KIT

8-OHdG top

8-hydroxy-2'-deoxyguanosine (8-OHdG / 8-oxo-dG) ELISA kit

"8-OHdG Check" is a competitive enzyme-linked immunosorbent assay for quantitative measurement of the oxidative DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA of tissue, urine, serum.

The characteristic features of this kit are:

1. Easy operation: No requirement of expensive equipments and sample pretreatment.
2. High sensitivity: Monoclonal antibodies recognize 8-OHdG specifically (21 analogues of 8-OHdG do not have any cross reactivity).
3. Fast: Estimated time of assay is 3.5 to 4 hours.
4. Sample capacity of one kit with a blank and standard is 18 for triplicate samples. Only 150l of sample is required (50l per well).
5. Split type microplates prevents excessive use of test samples.

Advantages of the "8-OHdG Check" Kit

The "8-OHdG Check"is useful as a non-invasive indicator in various fields as follows. In a primary evaluation of individual life style; in general, to learn whether individual antioxidant system are controlling excess active oxygen generated inside body properly and whether the oxidative stress is restrained within manageable limits, so that whether individual life style is in a state to prevent from diseases and to aging to enjoy the individual maximum life span.

2 In assessing the effect of antioxidant substance (food) in vivo.
3 In evaluating how much oxidative stress is generated by exercises performed or specific activities to which body is subjected to.
4 In studying the correlation between disease and oxidative stress.
5 In determining the side effect of oxidative stress following a specific clinical treatment procedure or pharmaceutical dose of medicine.
* Such techniques as HPLC-EC and LC-MS/MS which, by the nature of their procedure, measure only free form of 8-OHdG in biological samples, and can not detect 8-OHdG contained with in oligomers or 8-OHdG derivatives such as 8-OHdG holding SO3- or Glucuronic acid. ELISA technique is preferable to detect over all oxidative stress level in side body. (Referred by M.S.Cooke etc. Free Radical Research. 36(9), pp929-932 (2002)
Kit Contents
1. Microtiter plate coated with 8-OHdG (split type)
2. Primary antibody (Anti-8-OHdG monoclonal antibody)
3. Primary antibody solution
4. Secondary antibody (POD-conjugated anti mouse antibody)
5. Secondary antibody solution
6. Chromatic solution (3,3',5,5'-tetramethylbenzidine)
7. Substrate solution
8. Washing solution
9. Reaction terminating solution
10. Standard 8-OHdG solution (0.5, 2, 8, 20, 80, 200 ng/ml)
11. Plate seal
Assay Procedure
1. Primary Antibody Reaction (Competitive Reaction): 37°C for 1 hour
2. Secondary Antibody Reaction: 37°C for 1 hour
3. Development of Color Reaction: Room Temperature for 15 min in the dark
4. Absorbance Reading (wavelength at 450 nm) and Calculation of Results

Product List

Product Name Cat# Quantity Price

8-OHdG Check ELISA kit

NNS-KOG-200SE-EX 96WELL

¥ 102,400
$ 1366
€ 1024

References
  •  Cutler RG, Antioxidants and aging. Am J Clin Nutr. 1991 Jan;53(1 Suppl):373S-379S. PMID: 1985414
  •  Kasai H. et al, Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis. 1986 Nov;7(11):1849-51. PMID: 3769133
  •  Toyokuni S. et al, Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest. 1997 Mar;76(3):365-74. PMID: 9121119
  •  Saito S. et al, Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol. 2000;107(1-2):39-44. PMID: 11334369
  •  Nagayoshi Y. et al, Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events. Am J Cardiol. 2005 Feb 15;95(4):514-7. PMID: 15695143
  •  Morillas-Ruiz J. et al, The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol. 2005 Dec;95(5-6):543-9. Epub 2005 Aug 31.. PMID: 16132121
  •  Someya T. et al, Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats. Pediatr Nephrol. 2005 Oct;20(10):1430-4. Epub 2005 Aug 4. PMID: 16079985
  •  Shiihara T. et al, Acute encephalopathy with refractory status epilepticus: bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci. 2006 Dec 1;250(1-2):159-61. Epub 2006 Aug 28. PMID: 16935306