1. Oxidative stress causes diseases including cancer and aging.
Currently there is a strong scientific opinion that more than 90 percent of diseases are caused by oxidative stress due to active oxygen, other free radicals and lipid peroxides. Active oxygen, though vital to our body, causes considerable damage to the essential components of human body and disturbs the physiologically important functions of proteins, lipids, enzymes and DNA bearing the genetic code. This leads to disorder in our body, which in turn leads to development of diseases and accelerating aging. It has been demonstrated that oxidative stress causes cancers and many 'life-style related diseases such as arterial sclerosis, high blood pressure, myocardial infarction, cerebral apoplexy, dementia, diabetes, cataract etc.
2. What is 8-OHdG?
It has been calculated that about 2 percent of oxygen inhaled by the respiratory system turn to active oxygen in human body. This can reach to 10 percent in worse case scenario. Active oxygen is produced when our body generates energy and also when microphage cells confronts foreign, invading substances which may cause harm to the body and dispose them appropriately in our body system. Thus, active oxygen is essential and useful to our body. However, danger occurs when excess active oxygen is dispersed and which becomes detrimental to organs, body tissues and blood corpuscles.
Deoxyguanosine (dG) is one of the constituents of DNA and when it is oxidized, it is altered into 8-hydroxy-2'-deoxyguanosine (8-OHdG).
3. Balance of Active Oxygen and Anti-Oxidant System.
Human body is endowed with a counteracting defense system, to suppress excessive active oxygen so that body tissues and metabolic processes are prevented from disruption. This defense system is composed of components such as antioxidant enzymes, trace elements which aid the enzyme activity, vitamins and antioxidants referred to as super vitamins. As long as there exists a balance between the oxidative stress and antioxidant system, human body is maintained in an optimal health state. However, once the level of active oxygen released exceeds the protective capacity provided by the antioxidant system, human body suffers from the ill effects of oxidative stress. This leads to illness, death of cells and unstoppable excessive cell division resulting in cancer, and as a consequence acceleration of the aging process. Therefore, the maintenance of the delicate balance between oxidative stress and antioxidant system in proper order becomes very important.
4. How to check the balance between active oxygen and antioxidant system?
As one of the choice steps, we can measure the concentration and volume of human body components damaged by oxidation. The biomarker substance that aids in this detection should sensitively and proportionally respond to the degree of oxidative stress caused in the body, and should remain stable in the body until it is released and analyzed.
This resulting product is stable in the human body after it is excised from DNA by the repair enzyme system, and ultimately released into blood and excreted via urine. We can measure the concentration of 8-OHdG to determine the total oxidative stress (change) in our body which enables us primarily to evaluate our life style. Japan Institute for the Control of Aging (Dr. H.Ochi, Director of JaICA) researches with Prof.T.Osawa (Nagoya University) together and has established anti-8-OHdG monoclonal antibody. Even they have developed an original "8-OHdG Check" ELISA Kit using this antibody for the first time in the world.
|Details of "8-OHdG Check"|
5. The Science of Control of Aging.
The "8-OHdG Check" kit has another facet in its use in the control of aging process. For steps taken in scientific control of aging, it is necessary to determine the accelerative aging process and provide a feedback action. This means that when the progress of aging is assessed, one can take measures to retard the progress of aging by improving his or her life style or living environment. And detection of such measures facilitates in making further revision, if necessary. Since this check kit can determine the acceleration of degree of oxidative stress during aging, it serves to complete the aging control cycle and defines the science of aging control.
6. Completion of Individual Maximum Life Span in Good Health - to promote active life at least until 85 years.
The fundamental features of completing the individual maximum life span in good health is to establishing the life style, avoiding at most tasks which generate active oxygen, practicing optimum nutrition and optimum exercise. Optimum nutrition related to consuming well-balanced foods high with antioxidants. These guidelines ensures one to complete the allotted maximum life span and enjoy active life until 85 years. To find out whether one's current situation is appropriate or somewhat strayed from the optimal path, it pays to check one's 8-OHdG level at frequent intervals.
8-OHdG is useful as a general oxidative stress marker in the body. However, single marker is not sufficient for the analysis of oxidative stress in detail. For this purpose, we propose a screening system, named Oxidative Stress Profile (OSP). The OSP was developed by the joint efforts of JaICA. It evaluates the OSP in an individual's body by analyzing 80 endogenous components in blood, saliva and urine. The recommended strategy is for one to measure 8-OHdG first, and in case of extensive screening needed, employ the OSP analysis to find out the overall oxidative stress state of the body. Now we are developing new profiles for the human health in the world.