PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

useful to a general oxidative stress marker in the body.

DNA-damage Biomarker 8-OHdG "8-OHdG Check"

The 8-OHdG ELISA kit is a competitive in vitro enzyme-linked immunosorbent assay for quantitative measurement of the oxidative DNA adduct 8-hydroxy-2’-deoxyguanosine (8-OHdG) in tissue, urine etc. For research use only. Not for diagnostic nor medical use. Read entire insert before use.

Kit Contents (Highly Sensitive 8-OHdG Check #NNS-KOG-HS10E-EX)

  • 8-OHdG Microtiter plate : precoated with 8-OHdG (8™12wells, split type)
  • Primary antibody : monoclonal antibody specific for 8-OHdG
  • Primary antibody solution : phosphate buffered saline
  • Secondary antibody : HRP-conjugated antibody
  • Secondary antibody solution : phosphate buffered saline
  • Chromatic solution : 3,3',5,5'-tetramethylbenzidine
  • Diluting solution : hydrogen peroxide/citrate-phosphate buffered saline
  • Washing solution(™5) : 5 times concentrated phosphate buffered saline**
  • Reaction terminating solution: 1M phosphoric acid
  • Standard 8-OHdG solution : 0.125, 0.25, 0.5, 1, 4, 10 ng/ml 1vial each
  • Plate seal

*All reagent should be refrigerated at 2-8C.
*The kit should not be used beyond 9 months past the manufacturers date stamped on the exterior of the box.
**Dilute Washing solution by 5 times (v/v) with distilled water for use.

Summary of Assay Procedure

  1. The 8-OHdG monoclonal antibody and the sample or standard are added to the microtiter plate which has been precoated with 8-OHdG. The 8-OHdG monoclonal antibody reacts competitively with the 8-OHdG bound on the plate and the 8-OHdG in samples solution. Therefore higher concentrations of 8-OHdG in the sample solution lead to a reduced binding of the antibody to the 8-OHdG on the plate.
  2. The antibodies which are bound to the 8-OHdG in the sample are washed away from the antibodies that have bound to the 8-OHdG coated on the plate.
  3. An enzyme-labeled secondary antibody, which is added to the plate, binds to the monoclonal antibody which is bound to the 8-OHdG coated on the plate.
  4. Unbound enzyme-labeled secondary antibody is removed by a wash step.
  5. Addition of a chromatic substrate results in the development of color in proportion to the amount of antibody bound to the plate.
  6. The color reaction is terminated and the absorbance is measured.

Product List

Product Name Cat# Quantity Price

Highly Sensitive 8-OHdG Check ELISA kit

NNS-KOG-HS10E-EX 96WELL

¥ 102,400
$ 1366
€ 1024

8-OHdG Check ELISA kit

NNS-KOG-200SE-EX 96WELL

¥ 102,400
$ 1366
€ 1024

References
  • S.Okamoto, and H.Ochi Chemical Abst. 129859a (1992)
  • H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura, A.Ootsuyama, and H.Tanooka Carcinogenesis 7, 1849-1851 (1986)
  • S.Toyokuni, T.Tanaka, Y.Hattori, Y.Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa Lab.Invest. 76, 365-374 (1997)
  • M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert, and J.Lunec
    Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage.
    Biochemical and Biophysical Research Communications 259 pp374-378 (1999)
  • Tomoko Shimoike, Toyoshi Inoguchi, Fumio Umeda, Hajime Nawata, Katsumi Kawano and Hirotomo Ochi
    The meaning of serum levels of advanced glycosylation end products in diabetic nephropathy.
    Metabolism 49(8) pp1030-1035 (2000)
  • WenYing Fan, Kazunori Ogusu, Katsuyasu Kouda, Harunobu Nakamura, Tomoaki Satoh, Hirotomo Ochi and Hiroichi Takeuchi
    Reduced oxidative DNA damage by vegetable juice intake: A controlled trial.
    J Physiol Anthropol 19(6) pp287-289 (2000)
  • H.Ochi, M.Hashimoto, J.Kurashige
    Assessment of functional tea in human using oxidative stress profile technique.
    Proceedings of 2001 International Conference on O-Cha (tea) Culture and Science (2001)
  • M.S.Cooke, M.D.Evans and J.Lunec
    DNA repair: insights from urinary lesion analysis.
    Free Radical Research 36(9) pp929-932 (2002)
  • Tadashi Matsubasa, Takako Uchino, Shinnyo Karashima, Yuichi Kondo, Kenichi Maruyama, Masako Tanimura and Fumio Endo
    Oxidative Stress in very low Birth Weight Infants As Measured by Urinary 8-OHdG.
    Free Radical Research 36(2) pp189-193 (2002)