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Detection system For Human Tissue Sections

N-Histofine® Simple Stain AP

     Go to immunohistochemistry Polymer Detection System top

The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.

Feature and Advantages

N-Histofine Simple Stain AP is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by combining amino acid polymer with alkaline phosphatase (AP) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with human serum.

Principle & Procedure

Advantages

  • Simplified staining steps
  • High sensitivity
  • Unaffected by endogenous biotin in the tissue
  • Ready to use.

Staining Result

- Mouse anti-Muscle Actin antibody (clone:HHF35)

Human stomach staind with N-Histofine Simple Stain AP (M) and New fuchsin chromogen.
Note intense staining of smooth muscle in the walls of blood vessel and muscularis mucosae.

- Rabbit anti-S-100 protein antibody

Human colon staind with N-Histofine Simple Stain AP (R) and New fuchsin chromogen.
Note cyroplasmic staining of nerve cells scattered in smooth muscle and Auerbach's plexus.

 

Technical Information

NICHIREI Biosciences INC.

Product List

Product Name Cat# Quantity Price

N-Histofine Simple Stain AP (MULTI)

NIC-414261F 170TEST

¥ 40,000
$ 534
€ 400

N-Histofine Simple Stain AP (MULTI)

NIC-414262F 500TEST

¥ 80,000
$ 1067
€ 800

N-Histofine Simple Stain AP(M)

NIC-414241F 170TEST

¥ 40,000
$ 534
€ 400

N-Histofine Simple Stain AP(M)

NIC-414242F 500TEST

¥ 80,000
$ 1067
€ 800

N-Histofine Simple Stain AP (R)

NIC-414251F 170TEST

¥ 40,000
$ 534
€ 400

N-Histofine Simple Stain AP (R)

NIC-414252F 500TEST

¥ 80,000
$ 1067
€ 800

note : Please inquire about sales of these products to countries other than the United States of America.