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N-Histofine® - Technical Report

  Go to immunohistochemistry Polymer Detection System top

Recommendation of Immunohistochemical polymer detection systems designed mouse & rat tissue sections

Introduction

When the immunohistochemical detection systems for human tissue sections are used for staining on mouse and rat tissue sections, the background staining may be caused due to such reactivity with endogenous immunoglobulins of mouse and rat in the tissue. So that the immunohistochemical detection systems designed for staining on mouse and rat tissue sections were developed. In this report, the background staining is compared between detection systems for mouse, rat and human tissue sectoins on mouse/rat tissue sections.

Steps of immunohistochemical staining

Quenching of endogenous peroxidase

Incubation with PBS for 30 min.

Incubation with detection reagent for 30 min.

Incubation with DAB solution for 10 min.

Counterstaining

Mounting

Materials

  • Formalin-fixed paraffin-embedded mouse tissue sections

  • Formalin-fixed paraffin-embedded rat tissue sections

  • N-Histofine Simple Stain Mouse MAX PO (Rat) :
      a detection reagent designed for staining with a rat primary antibody on mouse tissue sections

  • N-Histofine Simple Stain Rat MAX PO (M) :
      a detection reagent designed for staining with a mouse primary antibody on rat tissue sections

  • N-Histofine Simple Stain MAX PO (M) :
      a detection reagent designed for staining with a mouse primary antibody on human tissue sections

Methods

To compare background staining between detection reagents, immunohistochemical staining on mouse tissue sections was performd with N-Histofine Simple Stain Mouse MAX PO (Rat) and N-Histofine Simple Stain MAX PO (M). (Fig.1)

To compare background staining between detection reagents, immunohistochemical staining on rat tissue sections was performed with N-Histofine Simple Stain Rat MAX PO (M) and N-Histofine Simple Stain MAX PO (M). (Fig.2)

PBS was used instead of a primary antibody in order to identify the background staining caused by detection reagents. DAB solution was used for brown color development.

 

Results

Fig.1 Mouse tissue sections

 

Background staining is not observed.

Background staining in plasma cells is observed.


Fig.2 Rat tissue sections

 

Background staining is not observed.

Background staining in plasma cells is observed.

NICHIREI Biosciences INC.