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Detection system for Rat tissue sections

N-Histofine® Simple Stain Rat MAX PO

  Go to immunohistochemistry Polymer Detection System top

The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.

Feature and Advantages

N-Histofine Simple Stain Rat MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded rat tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with rat, human, dog, pig and bovine sera.

Principle & Procedure

Advantages

  1. No reaction to endogenous mouse immunoglobulin in the tissue
  2. Simplified staining steps
  3. High sensitivity
  4. Unaffected by endogenous biotin in the tissue
  5. Ready to use

Staining Result

Mouse anti-Vimentin antibody (clone:V9)

Rat colon (treated with high temperature epitope unmasking method) stained with N-Histofine Simple Stain Rat MAX PO (M) and DAB chromogen.
Note cytoplasmic staining of fibroblasts and endothelial cells.

Mouse anti-PCNA antibody (clone:PC10)

Rat liver stained with N-Histofine Simple Stain Rat MAX PO (MULTI) and DAB chromogen.
Note nuclear staining of liver cell

Goat anti-CD3-ε(M-20)antibody

Rat lymph node (treated with high temperature epitope unmasking method) stained with N-Histofine Simple Stain Rat MAX PO (G) and DAB chromogen.
Note membrane staining of almost all lymphocytes.

Technical Information

NICHIREI Biosciences INC.

Product List

Product Name Cat# Quantity Price

N-Histofine Simple Stain Rat MAX PO (MULTI)

NIC-414191F 170TEST

¥ 35,000
$ 467
€ 350

N-Histofine Simple Stain Rat MAX PO (G)

NIC-414331F 170TEST

¥ 35,000
$ 467
€ 350