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Detection system for Mouse tissue sections

N-Histofine® Simple Stain Mouse MAX PO

    Go to immunohistochemistry Polymer Detection System top

The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.

Feature and Advantages

N-Histofine Simple Stain Mouse MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded mouse tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with mouseserum.

Principle & Procedure

Advantages

  1. No reaction to endogenous mouse immunoglobulin in the tissue
  2. Simplified staining steps
  3. High sensitivity
  4. Unaffected by endogenous biotin in the tissue
  5. Ready to use

Staining Result

Rat anti-Mouse CD45R/B220

Mouse spleen staind with N-Histofine Simple Stain Mouse MAX PO (Rat) and DAB chromogen.
Note membrane staining of almost all lymphocytes in germinal center and scattered interfollicular lymphocytes.

Rabbit anti-Keratin/Cytokeratin antibody

Mouse skin stained with N-Histofine Simple Stain Mouse MAX PO (R) and DAB chromogen.
Note cytoplasmic staining of epithelium cells and sweet gland cells.

Goat anti-CD3-ε(M-20)antibody

Mouse lymph node (treated with high temperature epitope unmasking method) stained with N-Histofine Simple Stain Mouse MAX PO (G) and DAB chromogen.
Note membrane staining of almost all lymphocytes.

 

Technical Information

NICHIREI Biosciences INC.

Product List

Product Name Cat# Quantity Price

N-Histofine Simple Stain Mouse MAX PO (R)

NIC-414341F 170TEST

¥ 38,000
$ 507
€ 380

N-Histofine Simple Stain Mouse MAX PO (G)

NIC-414351F 170TEST

¥ 38,000
$ 507
€ 380

N-Histofine Simple Stain Mouse MAX PO (Rat)

NIC-414311F 170TEST

¥ 38,000
$ 507
€ 380