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For delivering siRNA into animal tissues by local or systemic methods

in vivo siRNA transfection kit "AteloGene®" - Kit component

Kit component

Contents of kit
This kit is intended for 10 times of administration.

- Prefilled syringe (filled with “AteloGene
Each syringe is for 5 times of administration.
600 μL × 2 syringes
- 10 × siRNA buffer: 3 mL × 1 bottle
- Sterilized water: 3 mL × 1 bottle
- Microtube: 2 mL × 2 tubes
- Disposable syringe: 1 mL × 2 syringes
- 18G needle for ejection and suction: 4 needles
- 26G injection needle: 2 needles
- Instruction manual: 1 leaflet

 

Devices and reagents required other than the kit

- siRNA (PAGE or HPLC purified grade is recommended. Never fail to use siRNA preserved in water or in desalted and lyophilized condition.)
- Container for preparation of siRNA (sterilized, RNase free)
- Cooling device (crushed ice, cold block, etc.)
- Rotator (that can tumble and agitate. TAITEC RT-5, EYELA MBS-1A, etc.)
- Pipetter and tips (sterilized, RNase free)
- High-speed refrigerated centrifuge
- Anesthetic (as required)
- Mouse holder (as required)
- Cotton swab immersed in ethanol

 

Storage
Storage temperature: 2-10°C  (Do not freeze)
Effective period: 5 years from the manufacturing date indicated on the box
Precautions for storage

 

- Gelation and thermal denature occur in “AteloGene” at a temperature higher than 20°C. Never use “AteloGene” that was once gelated or thermally denatured.
- Freezing “AteloGene” causes bubbles in the mixture and the dispersion of components. Never use “AteloGene” that was once frozen.

 

Precautions for use

1)

This product is a reagent for research use only. Its application to the human body is strictly prohibited. Do not use it for any purposes other than for research purposes.

2)

Be sure to read the instruction manual before use. The manufacturer is not liable for the results of usage by methods other than that described in the instruction manual. Depending on the type of siRNA, administration target or administration method, the expected effect may not always be obtained.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.