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For delivering siRNA into animal tissues by local or systemic methods

in vivo siRNA transfection kit "AteloGene®" - Mechanism

Animation for understanding AteloGene

RNA interference (RNAi) is a mechanism in which fragments of double-strand ribonucleic acid (dsRNA) interfere with the expression of a specific gene whose sequence is complementary to the dsRNA. Recently, RNAi medicines, that inhibit the expression of genes responsible for diseases,have been studied actively. However, the delivery of siRNA in vivo had become a big issue because siRNA is unstable in vivo.

KOKEN(Tokyo) developed a new siRNA delivery system, “AteloGene” This system is mediated by atelocollagen that is a solubilized collagen obtained by pepsin treatment, and transfects siRNA in vivo efficiently and safety with each local administration and systemic administration.

 

Mechanism of RNAi and AteloGene

 

Overview
*Figures 1-5 indicate the mechanism of RNAi and figures 6-8 indicate AteloGene. System.


1.RNA polymerase II transcribes genomic DNA with mRNA.

2.RISC forms a complex with dsRNA.

3.Guide Strand/RISC complex binds the complementary mRNA

4.RISC complex cleaves the bound mRNA.

5.Cleaved mRNA fragments are degenerated by RNase.

6.Atelocollagen forms complex with dsRNAs electrostatically and taken up by Endocytosis

7.Atelocollagen protects dsRNAs from digestion by RNase.

8.dsRNAs are dissociated from atelocollagen and initiate RNAi.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.