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one step culture cell storage solution.

COS Banker Instructions

Background

Cos Banker is a one step culture cell storage solution. Suspend culture cells in Cos Banker and store at -80 degrees for long term storage (it is not necessary to store in liquid nitrogen). This is a serum-free solution with no animal products added.


COS banker series flyer download [PDF]

Feature and Advantages

  • Ready to Use (No Dilution Required!)
  • Possible to store the Cells at -80℃ in Deep freezer
  • Possible to retain Cells in high Viability after defrosting
  • Does not contain Serum or Animal products*
  • Endotoxin-free tested / Bacteria free tested
   * "Cos Banker II" contains Human Serum Albmin (GMP Grade) as Stabilizer

Kit component

Cos Banker (120 ml)

  • Contains DMSO 10%
  • No serum
  • Store at 2-10 degrees of dark place
  • Expiration date: 2 years from date of manufacture

Applications tested

  • P3UI (mouse myeloma cells)
  • OKT4 (mouse hybridoma cells)
  • PC12 (human neuronal cells)
  • K562 (human leukemia cells)
  • Other human and mouse lymphocytes

Instructions

  1. Collect culture cells (during logarithmic growth stage) and place 5x105 - 1x107 cells in a cryovial. Centrifuge for 5 minutes at 1,200 rpm.
  2. Add 1 ml of Cos Banker to cell pellet and vigorously mix, then freeze and store at -80°C.
  3. If liquid Nitrogen storage is desired, freeze cells at -80°C first and then transfer to liquid Nitrogen.
  4. To thaw, defrost in 37°C water bath quickly.

Note: It is ideal to use a logarithmic growth stage culture in order to increase the survival rate when thawed.

Product List

Product Name Cat# Quantity Price

COS banker [Cell Freezing Medium] (chemical difined)

KOJ-COS-CFM01 120ML

¥ 12,000
$ 160
€ 120

COS banker II [Cell Freezing Medium]

KOJ-COS-CFM02 120ML

¥ 12,000
$ 160
€ 120

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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