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Anti 5-Fluorouracil antibody

Background

5-Fluorouracil (5-FU) is a pyrimidine analogue and inhibits an enzyme called thymidylate synthetase, which results in inhibition of DNA replication. Thus, 5-FU is used as a drug in the treatment of cancers including colorectal cancer, pancreatic cancer and skin cancer.

Specification

Host Mouse
Specificity 5-FU (both free 5-FU and protein-bound 5-FU)
Antigen 5-Fluorouridine-BSA(5-FU-BSA)[5-FU]/[BSA]=10.2
Clone H3-17
Subclass IgG1, λ
Cross reactivity The antibody does not have cross-reactivity with Uracil, Cytosine, Thymine,
5,6-Dihydro-5-methyluracil and 5,6-Dihydrouracil.
Applications ELISA (Other applications have not been tested.)

Example Assay Data



Fig.1 Monoclonal antibody (H3-17) shows high binding to 5-FU-BSA but undetectable binding to BSA.
Different dilutions of antibody were tested for binding to immobilized antigens (100 ng / well) in a direct ELISA.




Fig.2 Monoclonal antibody (H3-17) is capable of binding to free 5-FU..
Free 5-FU efficiently inhibits the antibody binding to immobilized 5-FU-BSA
(5 ng / well), which was detected by a competitive ELISA.




Fig.3 Binding specificity of Anti-5-FU antibody
Anti-5-FU monoclonal antibody (H3-17) binds to 5-FU, but not to Uracil,
Cytosine, Thymine,5,6-Dihydro-5-methyluracil and 5,6-Dihydrouracil.

Product List

Product Name Cat# Quantity Price

Anti 5-Fluorouracil

CAC-NM-MA-002 1VIAL

¥ 40,000
$ 534
€ 400

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.