5-Fluorouracil (5-FU) is a pyrimidine analogue and inhibits an enzyme called thymidylate synthetase, which results in inhibition of DNA replication. Thus, 5-FU is used as a drug in the treatment of cancers including colorectal cancer, pancreatic cancer and skin cancer.
|Specificity||5-FU (both free 5-FU and protein-bound 5-FU)|
|Cross reactivity||The antibody does not have cross-reactivity with Uracil, Cytosine, Thymine,
5,6-Dihydro-5-methyluracil and 5,6-Dihydrouracil.
|Applications||ELISA (Other applications have not been tested.)|
Fig.1 Monoclonal antibody (H3-17) shows high binding to 5-FU-BSA but undetectable binding to BSA.
Different dilutions of antibody were tested for binding to immobilized antigens (100 ng / well) in a direct ELISA.
Fig.2 Monoclonal antibody (H3-17) is capable of binding to free 5-FU..
Free 5-FU efficiently inhibits the antibody binding to immobilized 5-FU-BSA
(5 ng / well), which was detected by a competitive ELISA.
Fig.3 Binding specificity of Anti-5-FU antibody
Anti-5-FU monoclonal antibody (H3-17) binds to 5-FU, but not to Uracil,
Cytosine, Thymine,5,6-Dihydro-5-methyluracil and 5,6-Dihydrouracil.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.