|100mM Tris (pH 7.4) with 0.02% Sodium Azide|
|Storage||Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
|Purification method||Gel filtration chromatography|
western blotting : 3-10μg/ml,
Immunohistochemistry (IHC, IHC-P): 1-10 ug/ml
Flow cytometry: 1-5 ug/ml (Permeabilization is recommended)
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
Figure 1: Immunohistochemistry of CD204 in human colon cancer and lymphoma (Paraffin sections).
There are many CD204-positive macrophages in stroma of colon cancer (left) and lymphoma (right). Antigen retrieval: 1mM EDTA buffer (pH8.0), heat (microwave for 5 min)
Figure 2: Western blot analysis of CD204 in human monocyte-derived macrophages.
Predicted molecular weight of CD204 is 43 kDa, however, molecular mass is approximately 50-70 kDa due to glycosylation under reducing condition.
Pre-treatment with N-glycosidase is recommended.
Lane 1: Control human monocyte-derived macrophages , Lane 2: Macrophages stimulated with IL-4, Lane 3: Macrophages stimulated with IL-10, Lane 4-6: Macrophages stimulated with conditional medium of breast cancer cell lines.